FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39192473
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC15600.08.8451235
CCGTCCG17400.07.731855434
GTCCGCA19000.06.899220536
ACCCGTC21500.06.738747632
CACGACG10251.6211197E-76.731230315
TATGCCG13753.616151E-96.27339548
GATCGGA79050.06.19828869
CGTCTGA41200.06.112313716
GCGTGAG124800.06.053379522
CGTGAGC130100.06.01889723
CGTTGGA88100.05.99379251
AGATCGG90650.05.9760969
ACAGGCG181850.05.956495318
CCCAAAG537000.05.8550451
ACGACGC8952.9030856E-45.781602416
TCCGCAT23300.05.77404837
CACCCGT28250.05.739152431
ACACGTC43500.05.710113
GAGCCAC384400.05.563865726
CCAAAGT508100.05.54094172
Produced by FastQC (version 0.11.5)