FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26950079
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG404400.06.87017441
GCCACCG97400.06.304289328
CCAAAGT386450.06.0264022
GAGCCAC293700.06.025436426
ACAGGCG138800.05.939970518
GGATTAC371750.05.929814313
GATCGGA19255.456968E-125.91651871
GGGATTA400250.05.800626812
CCCAAAT225000.05.7981881
CCCTAAA121250.05.7497991
TACAGGC344800.05.71276517
TTACAGG409050.05.684111616
CGTTGGA72950.05.62995151
ACCGCGC37650.05.58907531
ACCCGGC46400.05.57598536
CCCAAGT170450.05.568251
GCGTGAG102400.05.55851922
CGTGAGC105900.05.537682523
AAAGTGC339600.05.5268244
CAAAGTG388850.05.45677143
Produced by FastQC (version 0.11.5)