FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33171228
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCG163900.07.177600418
CGTGAGC116750.06.737307523
CGTTGGA76450.06.5430941
GCGTGAG116950.06.460313322
ACGGGAT49900.06.4289459
CCCAAAG449800.06.2890791
ACCGCGC39350.06.22461131
TACAGGC390300.05.904436617
CCAAAGT429300.05.83317332
CAGGCGT151750.05.7971619
AACGGGA70250.05.79415858
GAGCCAC328750.05.71917326
GGATTAC419050.05.71321313
AGGCGTG158300.05.6227320
GGGATTA454100.05.522732312
CGCCCGG73000.05.48214735
AAACGGG89400.05.47902067
CGGCTAA64250.05.47719739
TTACAGG458900.05.40498316
CCGAGTA78550.05.4011732
Produced by FastQC (version 0.11.5)