FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30749412
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11400.011.80370148
CGTATGC14550.08.77319146
CTCGTAT15350.08.09103144
TGCCGTC18400.07.50088850
CCCAAAG409150.07.1452521
CGTCTGA31500.07.008939316
CGTTGGA73100.06.9409141
CGTGAGC95500.06.935400523
ACAGGCG135500.06.87387618
TCTCGTA16700.06.817173543
GCGTGAG97350.06.732732322
GCCACCG92850.06.650344428
CCAAAGT394250.06.32739642
ACACGTC35500.06.219351313
ACCGTGC41300.06.18097231
AACGGGA65650.06.14805278
AAACGGG79300.06.1337397
ACGTCTG40300.06.07773615
GAGCCAC286850.06.06106226
GCCGTCT22400.06.007446351
Produced by FastQC (version 0.11.5)