FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32931730
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG128400.07.871814722
CCCAAAG502950.07.64455561
CGTGAGC128500.07.59723523
ACGGGAT60750.07.49596559
ACAGGCG176900.07.312698418
CGTTGGA89700.07.31060081
GCCACCG118000.07.250065328
CGGGATT58900.07.145694710
CCAAAGT481300.07.03226572
ACCGTGC48700.06.800087531
ACCGCGC46500.06.75088531
CCCAAAA255750.06.74755141
GAGCCAC360650.06.580740526
GGATTAC465250.06.562340713
TACAGGC426750.06.426440217
CCCAAGT210550.06.4257331
CCCTAAA151350.06.40790131
CCCAAAT266700.06.3928691
AAAGTGC419550.06.38971424
GGGATTA500900.06.336314712
Produced by FastQC (version 0.11.5)