FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26944724
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11600.011.00433546
CGATCAG15950.08.21859933
CGTATGC14450.08.11755944
ACGACGC5958.891286E-68.11698716
CGACGCT6251.7151353E-57.727314517
CTCGTAT15300.07.66631442
GTCACGA17400.07.533703329
TCTCGTA16600.07.273504741
TCGTATG17700.07.016808543
TGCCGTC19000.06.71910948
ATGCCGT21500.06.419083647
GCGTGAG89050.06.120646522
CGTGAGC89550.06.08647223
ACAGGCG124750.06.083606218
CCCAAAG362450.05.9515841
GCCGTCT20300.05.948907449
TCACGAT25450.05.82847330
AGATCGG64400.05.6792869
GGATTAC326900.05.624824513
CCAAAGT347950.05.6222332
Produced by FastQC (version 0.11.5)