FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26944724
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCG124000.06.204181718
GCGTCGT21700.06.200483369
CGTTGGA57200.06.03135541
ACGGGAT38350.05.84674029
AGATCGG63400.05.71374369
CGTGAGC88900.05.627045623
GCGTGAG86250.05.599905522
CCCAAAG351700.05.571691
CGTCGTG21705.456968E-125.564608668
AGCGTCG24850.05.55341268
CCGAGTA56550.05.48967652
GAGCCAC250500.05.384889626
TACAGGC299450.05.36861317
ACCACGC74800.05.35005831
GGATTAC315050.05.255956613
ACCGCGC32900.05.24295631
AAACGGG66200.05.1586757
AACGGGA53550.05.1534018
CCAAAGT333100.05.1155122
GGGATTA346200.05.09182412
Produced by FastQC (version 0.11.5)