FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31516563
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12250.08.44902246
CCCAAAG472850.07.52502871
ACAGGCG174850.06.94473318
CGTGAGC125300.06.91031323
CCAAAGT452200.06.85912942
GCGTGAG120600.06.77916422
CCCAAGT203700.06.55681371
CCCATAA129200.06.51778751
GATCGGA37300.06.38279869
GAGCCAC341600.06.362040526
GCCACCG119150.06.224695728
GGATTAC448450.06.192714713
AAAGTGC393800.06.14163834
CCCAAAA247750.06.1014541
TACAGGC420050.06.04446917
CGTTGGA84600.06.0375991
GTGCGTT37300.06.01266648
CTCGTAT15751.9354047E-95.914034442
ACACGTC26850.05.91034413
ACCGTGC46750.05.903126731
Produced by FastQC (version 0.11.5)