FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31516563
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA88900.07.41161161
CCCAAAG485450.07.1488231
GCGTGAG122250.07.111439722
CGTGAGC124550.07.063268723
GATCGGA35950.06.909770569
AGCGTCG14301.9281288E-106.514149769
CCAAAGT458300.06.41992432
ACGGGAT62200.06.37774479
ACAGGCG170700.06.36612718
AAACGGG102750.06.3115457
CCCATAA129350.06.2673291
ACCGTGC49650.06.11459831
GCCACCG115850.06.10447828
GAGCCAC347750.06.091307626
GGATTAC444050.05.99745713
GAAACGG109850.05.9663176
AAAGTGC390100.05.959694
AACGGGA84950.05.92856848
TACAGGC408850.05.89807217
AGATCGG42150.05.893386469
Produced by FastQC (version 0.11.5)