FastQCFastQC Report
Wed 8 May 2024
EGAF00004834049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14993821
Total Bases764.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC5171373.4490007583790687RNA PCR Primer, Index 1 (100% over 50bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC2469751.6471785277415278RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2087551.392273523873601RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG1881821.255063669227477No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1628951.0864141968881715RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1608561.0728152617001363No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1262390.8419401565484876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC1252690.8354708249484903No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1138110.7590526791002774RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC1101670.7347493344091542No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1076950.7182625429501927No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT893500.5959121427420002No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA839050.5595971833997485RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA824510.5498998554137735RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA817810.545431348019961No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC801460.5345268560962546RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA777530.5185669483449216RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA685180.45697490986453687RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA654690.43663986651568RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC625550.4172051940596063RNA PCR Primer, Index 1 (100% over 27bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA604080.4028859621573446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT580680.38727953334910425No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA575400.38375808274621925No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC537930.3587677884109728No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT512640.341900840352836RNA PCR Primer, Index 1 (100% over 35bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCA502180.3349246332872721RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC454480.3031115284089359RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC446640.29788270781677334RNA PCR Primer, Index 1 (100% over 31bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCC446330.29767595598213425RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG437660.2918935740262606No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG429290.28631127449100535No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC429070.28616454738255176RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA409630.27319920652647517No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA407680.2718986707924551RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA402950.26874403796070395RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG387620.2585198262671003No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG383470.2557520194485448No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG378430.2523906347821546No Hit
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGG376630.2511901402584438No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC366970.24474748631452917RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA362730.24191965476978816RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC356000.23743113913391392RNA PCR Primer, Index 1 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA353820.2359772068774197RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG342980.22874756207907246Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC335100.2234920638308274No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA330910.22069757935618944RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA330390.22035076982711743RNA PCR Primer, Index 1 (100% over 37bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGG326850.21798979726381953No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC319460.21306110030258466RNA PCR Primer, Index 1 (100% over 27bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC319090.21281433198382188RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA310230.2069052311615565RNA PCR Primer, Index 1 (100% over 34bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC306610.20449090328609362No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC303320.20229666607331112RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC301050.2007827090906314RNA PCR Primer, Index 1 (100% over 27bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA300350.2003158501091883RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA299220.19956220632485877RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC297110.1981549599665089RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT282020.1880908142094RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC275220.18355561267538142RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC269930.18002749265847579RNA PCR Primer, Index 1 (100% over 27bp)
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG267530.17842683329352807No Hit
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT249160.16617511973765728No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC245330.1636207341677615RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC234630.15648446116570286RNA PCR Primer, Index 1 (100% over 27bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGC234210.156204345776837No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA232420.15501052066714682RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA227930.15201595377189045RNA PCR Primer, Index 1 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT227640.15182254076529259No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC226870.1513089958857052RNA PCR Primer, Index 1 (100% over 31bp)
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCT218590.14578672107663548No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA218080.1454465809615841RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA217080.14477963955952255RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAT212740.14188511387457542No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC210280.140244438025504RNA PCR Primer, Index 1 (100% over 26bp)
ACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAAC207950.13869046455870054RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC205330.13694307808529926RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCA203070.13543579051664015RNA PCR Primer, Index 1 (100% over 28bp)
GGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCC197670.13183430694550777RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC196800.1312540679257142No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA195210.13019363109643634RNA PCR Primer, Index 1 (100% over 32bp)
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC189310.12625867682427314RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC187580.12510486819870664No Hit
AACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAA186270.12423117496200602RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG184380.12297065571210967RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA182260.12155673993973917No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA179520.11972932049809051No Hit
GGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTC179230.11953590749149268No Hit
TGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCCAAG179070.1194291968671628No Hit
GGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCG176930.11800194226675108No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC176860.11795525636860678RNA PCR Primer, Index 1 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT174160.11615451458304056RNA PCR Primer, Index 1 (100% over 25bp)
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC170420.11366015373933036RNA PCR Primer, Index 1 (100% over 27bp)
TCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCG167160.11148592476860969No Hit
GTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGT166030.11073228098428013No Hit
AAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGA165420.11032544672902257No Hit
AGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA163690.10917163810345608No Hit
TGAGGTAGTAGATTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA162320.10825792838263175RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG162250.10821124248448745No Hit
TGGTAGACTATGGAACGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG161060.10741758221603419RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG159630.10646385601108617No Hit
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC154830.1032625372811907RNA PCR Primer, Index 1 (100% over 33bp)
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA154230.10286237243995378RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC151100.10077484585150111RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)