FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004834049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14993821
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC5171053.4487873371304087RNA PCR Primer, Index 1 (100% over 51bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC2456401.6382748600240058RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC2076221.3847170777882436RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG1863651.2429453439520186No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1626981.0851003223261102RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG1607341.0720015931896212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1261600.841413272840859No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC1249930.8336300666788007No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1137050.7583457212140922RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT1101090.7343625083959586No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1076120.7177089815864816No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG892510.5952518707539592No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG838280.5590836385201611RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG823880.5494796823304747RNA PCR Primer, Index 1 (100% over 29bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC817270.5450711996628478No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC797250.5317190327935755RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG776950.5181801223317258RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC684780.4567081333037123RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG654090.4362397016744431RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA624860.4167450044921838RNA PCR Primer, Index 1 (100% over 28bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA603540.40252581380023145No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC579580.38654589780683657No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA575010.38349797559941523No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA537710.3586210613025192No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC512570.3418541544546917RNA PCR Primer, Index 1 (100% over 36bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG501600.33453780727407645RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC451960.30143083607574084RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA446490.2977826666064641RNA PCR Primer, Index 1 (100% over 32bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA445770.2973024687969798RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGC437360.29169349160564206No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC429000.2861178614844075No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA428880.28603782851616005RNA PCR Primer, Index 1 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA409300.2729791158637948No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG407300.2716452330596717RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC402760.26861731909431225RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC387370.2583530909165849No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG379540.2531309397384429No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGC377150.25153694978751584No Hit
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG376020.2507833060031862No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA366400.24436732971535408RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG362480.24175291941927277RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA355770.23727774261143972RNA PCR Primer, Index 1 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG353550.2357971326988631RNA PCR Primer, Index 1 (100% over 29bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGGA342760.2286008349706189RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC334270.2229385024671163No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG330720.22057086048979774RNA PCR Primer, Index 1 (100% over 29bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC330390.22035076982711743RNA PCR Primer, Index 1 (100% over 38bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGG326290.21761631007866508No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA319180.21287435671000743RNA PCR Primer, Index 1 (100% over 28bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA318740.21258090249310033RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT310200.20688522291949463RNA PCR Primer, Index 1 (100% over 35bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG305830.20397068899248566No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA302920.20202988951248652RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA300770.20059596549805414RNA PCR Primer, Index 1 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG300040.20010909827454923RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG299050.19944882628650829RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC295410.19702115958300423RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC281130.1874972363615652RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC273710.18254853115826844RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA269670.17985408789393978RNA PCR Primer, Index 1 (100% over 28bp)
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT267300.1782734367710539No Hit
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTG248890.16599504555910063No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA245080.16345399881724612RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA234350.15629771757312563RNA PCR Primer, Index 1 (100% over 28bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCC233740.15589088331786807No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG232230.15488380180075512RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG227740.15188923490549874RNA PCR Primer, Index 1 (100% over 29bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC227510.15173583838302457No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA226810.1512689794015815RNA PCR Primer, Index 1 (100% over 32bp)
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTG217980.14537988682137795No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG217900.14532653150921304RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG216900.14465959010715146RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATT212530.1417450561801425No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC209210.1395308107252981RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAACT207800.1385904233483913RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC204340.13628280609725832RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG202890.13531574106426908RNA PCR Primer, Index 1 (100% over 29bp)
GGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA197510.13172759632117792RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC196300.1309205972246834No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC195110.13012693695623018RNA PCR Primer, Index 1 (100% over 33bp)
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC188130.1254716859698405RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCC187200.12485143046592326No Hit
AACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAAC186160.12415781140777925RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT184200.12285060625973859RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA182110.12145669872942995No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG179410.11965595694386373No Hit
TGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCCAAGG179000.1193825109690185Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCG178970.11936250272695666No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG176850.11794858695458615RNA PCR Primer, Index 1 (100% over 39bp)
GGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGG176780.11790190105644185No Hit
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTC173570.11576101915582425RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA170270.11356011252902111RNA PCR Primer, Index 1 (100% over 28bp)
TCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGG167020.11139255297232106No Hit
GTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTG165910.11065224801603273No Hit
AAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA165380.11029876907294012No Hit
AGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAT163660.10915162986139425No Hit
TGAGGTAGTAGATTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG162190.10817122600036375RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT162060.10808452361809574No Hit
TGGTAGACTATGGAACGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT160930.10733087983376619RNA PCR Primer, Index 1 (100% over 30bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC159550.10641050069892125No Hit
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA154770.10322252079706701RNA PCR Primer, Index 1 (100% over 34bp)
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG154150.10280901712778884RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA151060.10074816819541862RNA PCR Primer, Index 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)