FastQCFastQC Report
Fri 10 May 2024
EGAF00004834052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14374194
Total Bases733 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC4456803.1005564555480465RNA PCR Primer, Index 5 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG2661851.8518255701850135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2301861.601383701931392No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2214811.5408237846240282RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC1732871.2055423768456166RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1725311.2002829515171425RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT1703601.1851794959773048No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1344450.9353220083157359No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1302880.9064021259209385No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC1263480.878991893388944No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1135160.789720801041088RNA PCR Primer, Index 1 (100% over 26bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1097740.7636880370475033No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG875680.6092028533912928No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC818160.5691866966593049No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG812470.565228213839329No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG765160.5323150640655052No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG727790.5063170846309712No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA707650.4923058642453274RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA664970.46261376463960346No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC660580.4595596803549472No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG645870.44932606308221523No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC581360.40444702499493185RNA PCR Primer, Index 1 (100% over 27bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGG572240.39810232142407426No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA513000.35688957586074044RNA PCR Primer, Index 1 (100% over 28bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC512890.3568130498308288No Hit
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGG503230.350092673022223No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA475570.33084985495534563No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA459280.3195170456166099No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA457220.3180839217837188No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA433100.30130385049763486No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC402010.2796748116798758No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC399850.27817211872888314RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC396400.27577198415438114RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG381540.2654340132044969No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATTGGAATTCTCGGGTGCCAAG370460.2577257549188497No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC367000.2553186634325375No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC354560.24666426514070983No Hit
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG351220.24434065659611942No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA345090.2400760696564969RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA341240.23739765860958884RNA PCR Primer, Index 1 (100% over 28bp)
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCT329490.22922328723266155No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC325490.22644052250860117RNA PCR Primer, Index 1 (100% over 31bp)
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAT320400.22289945439723438No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC316520.22020017261489586RNA PCR Primer, Index 1 (100% over 27bp)
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT315380.21940708466853864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT302990.2107874709357617No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA300160.208818664893489RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA297670.20708639385276142RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA292690.20362185177130626RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG288290.20056081057483988RNA PCR Primer, Index 1 (100% over 29bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGC286250.1991416005655691No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC280590.19520398848102372RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC273450.190236753448576RNA PCR Primer, Index 1 (100% over 27bp)
GTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGT272030.18924887197153453No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG267700.18623652915773922Illumina Small RNA Adapter 2 (100% over 21bp)
GGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCG252960.1759820411495768No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC251400.17489676290719328RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA249800.17378365701756912No Hit
AAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGA245430.1707434865565332No Hit
TCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCG240010.1669728403554314No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC239700.16675717608931673No Hit
GGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTC236030.16420398945499137No Hit
AGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA235820.1640578943069782No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA234320.16301435753545557RNA PCR Primer, Index 1 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA234040.16281956400477132RNA PCR Primer, Index 1 (100% over 28bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTTGGAATTCTCGGGTGC232430.16169950120333704No Hit
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC225540.15690618896614308RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCA225090.1565931279346863RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC223760.15566785866393623RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC221170.15386601850510714No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC218290.15186242790378368No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG217240.15113195216371783No Hit
ACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAAC213120.14826570449793566RNA PCR Primer, Index 1 (100% over 24bp)
AAAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGG210030.14611601874859906No Hit
CGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTG207630.14444635991416285No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA207250.14418199726537712RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC206600.1437297979977173RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG203690.14170533666096338RNA PCR Primer, Index 1 (100% over 29bp)
TGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCCAAG203430.14152445695389948No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC200710.13963217694153843RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG199310.13865820928811728No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA197410.13733639604418862RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA196160.13646678206791976RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC195410.13594501368215844No Hit
AACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAA193080.13432405323039331RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT181850.12651144126759384RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC181260.12610098347079496RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC181130.126010543617263RNA PCR Primer, Index 1 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC178110.12390955625059742RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA178090.12389564242697712RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC176260.12262252756571951No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC176210.12258774300666876RNA PCR Primer, Index 1 (100% over 27bp)
AAAAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTG175900.12237207874055407No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA175680.12221902668073076RNA PCR Primer, Index 1 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT172810.12022239299121745No Hit
GATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACG169800.11812836253636205No Hit
TTGTGGGAACAATGGCACGGCAAGGGGCTCAGTATTTTTGGAATTCTCGG169350.11781530150490524No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA163160.11350897309442184No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC161920.11264631602996315RNA PCR Primer, Index 1 (100% over 26bp)
ACACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAG161360.1122567289685947No Hit
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC156540.10890349747610198RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG155400.10811040952974477No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG150490.10469456583096068No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAA149190.10379016729564106No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA148860.10356058920590609RNA PCR Primer, Index 1 (100% over 28bp)
ACGGCCATGAGATCAACCCCATGCACCGCTCTGAGACCTTGGAATTCTCG147800.10282315655403008No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC146820.10214137919663531RNA PCR Primer, Index 1 (100% over 26bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCC146720.10207181007853379RNA PCR Primer, Index 1 (100% over 27bp)
AGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCT145010.10088217815899798No Hit
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTC144100.10024909918427427RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)