FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004834052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14374194
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC4456583.1004034034882237RNA PCR Primer, Index 5 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG2629211.8291182100366812No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA2298791.5992479300056754No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2212221.5390219444651994RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1723201.1988150431252005RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC1721571.197681066500146RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG1701491.183711587585363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC1341790.9334714697742356No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1302190.9059220990060383No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC1260520.8769326474931394No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1127830.7846213846842474RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG1096670.7629436474838172No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGC874750.6085558605929487No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT817620.5688110234215566No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG803370.5588974240920916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC764410.5317932956797439No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC727110.505844014627881No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG706880.49177018203594586RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA664100.4620085133121203No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA660120.4592396624116803No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGC644220.4481781726335404No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA580460.40382090293201833RNA PCR Primer, Index 1 (100% over 28bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGG571200.3973788025958186No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG512350.3564373765930806RNA PCR Primer, Index 1 (100% over 29bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG511690.35597822041361066No Hit
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG502330.34946655095930945No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA474910.33039069877587574No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA458790.3191761569379125No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC456790.3177847745758823No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA432400.3008168666709243No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCC401210.2791182587350637No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC397500.2765372444534977RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC393800.2739631870837419RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT381130.2651487798202807No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAATTGGAATTCTCGGGTGCCAAGG370240.2575727028590264Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA366800.25517952519633447No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC353680.24605205690141652No Hit
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT350840.24407629394733368No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG344700.23980475009590102RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG340800.23709155448994218RNA PCR Primer, Index 1 (100% over 29bp)
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTG328410.22847194075716523No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA325320.2263222550078286RNA PCR Primer, Index 1 (100% over 32bp)
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATT319970.22260030718939788No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA316050.21987319775981876RNA PCR Primer, Index 1 (100% over 28bp)
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTG314980.21912880819613262No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG302570.21049528063973535No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG299930.20865865592185553RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC297540.20699595399922946RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG292350.20338531676976113RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT288020.2003729739559658RNA PCR Primer, Index 1 (100% over 30bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCC285680.1987450565923905No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA280150.19489788436137706RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA272930.18987499403444813RNA PCR Primer, Index 1 (100% over 28bp)
GTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTG271840.18911669064714168No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGGA267500.1860973909215362RNA PCR Primer, Index 1 (100% over 22bp)
GGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGG252710.17580811835432303No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA251140.17471588320012935RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG249650.17367930334041687No Hit
AAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAA245310.17066000361481137No Hit
TCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGG239900.16689631432551974No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA239550.16665282241216448No Hit
GGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCG235840.1640718081305985No Hit
AGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAT235710.16398136827706652No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG234060.1628334778283916RNA PCR Primer, Index 1 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG233760.1626247704740871RNA PCR Primer, Index 1 (100% over 29bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTTGGAATTCTCGGGTGCC231940.16135861252463962No Hit
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA225210.15667661087640808RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG224880.15644703278667313RNA PCR Primer, Index 1 (100% over 29bp)
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA223350.15538262527972005RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTCG220900.15367818188623306No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA218190.1517928587856822No Hit
ACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAACT212820.14805699714363116RNA PCR Primer, Index 1 (100% over 25bp)
AAAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGA209940.1460534065423077No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG207060.14404981594098423RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA206290.14351413373160263RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT203520.14158706916019081RNA PCR Primer, Index 1 (100% over 30bp)
TGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCCAAGG203240.1413922756295066Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC199460.13876256296526956RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG199180.13856776943458535No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG197140.13714855942531456RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG195950.13632068691990662RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT194280.1351588826476114No Hit
AACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAAC192940.1342266564650512RNA PCR Primer, Index 1 (100% over 24bp)
CGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGT184150.12811153098392855No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC181300.12612881111803556RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA181010.12592706067554119RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA180830.12580183626295846RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG177890.12375650419077411RNA PCR Primer, Index 1 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC177020.12315125286329097RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA175880.12235816491693377RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC175850.12233729418150333No Hit
AAAAGTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGG175830.12232338035788302No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG175450.12205901770909729RNA PCR Primer, Index 1 (100% over 29bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC172700.1201458669613058No Hit
TTGTGGGAACAATGGCACGGCAAGGGGCTCAGTATTTTTGGAATTCTCGGG168950.11753702503249921No Hit
GATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG163240.11356462838890305No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAA163120.11348114544718126No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC160890.11192975411351759RNA PCR Primer, Index 1 (100% over 27bp)
ACACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGC160880.11192279720170745No Hit
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC155420.10812432335336507RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC155270.1080199696762128No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAGG150130.10444411700579524No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAAT149120.10374146891297001No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG148730.10347014935237413RNA PCR Primer, Index 1 (100% over 29bp)
ACGGCCATGAGATCAACCCCATGCACCGCTCTGAGACCTTGGAATTCTCGG147700.10275358743592859No Hit
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA146520.10193267184233076RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC145850.10146655875105066RNA PCR Primer, Index 1 (100% over 27bp)
AGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTC144740.10069434154012391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)