FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004834053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11225161
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC4683864.172643938024586RNA PCR Primer, Index 6 (100% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2980582.6552670380407015RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2247312.002029191385317RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC1816541.6182752300835597RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1356291.2082588392273395RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1244171.1083760847617241No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG1034830.921884327538821No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1032780.9200580731091519No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG923630.8228211604270086RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC779050.6940212260652654No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA724630.6455408523761931RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG631130.5622458332668903RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG573530.5109325380722824No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG558480.4975251579910524RNA PCR Primer, Index 1 (100% over 29bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGG534490.47615352688482593No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA522330.46532072012151987No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC477970.42580235597511695RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC476490.4244838893624777RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG442320.39404334601525987RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA438600.39072936236727474RNA PCR Primer, Index 1 (100% over 28bp)
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA430420.38344216176498497RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG405960.3616518284236636RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT404460.36031554469463734RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG400050.35638687053130014RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA393160.3502488739359729No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC386170.3440217917587106No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC376820.3356922898477803RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC376480.3353893988692011RNA PCR Primer, Index 6 (100% over 36bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA371170.3306589544684482RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA330830.29472183071583563RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG329560.29359044382526006No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG324400.2889936277974098No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA322150.2869892022038704RNA PCR Primer, Index 1 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG313300.2791051282026155RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA311490.27749267916959053RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA310930.27699379991075407RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG306220.2727978690016117RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC302540.2695195195864006No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA296550.2641832932284891No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG290420.2587223470558685RNA PCR Primer, Index 6 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC289560.2579562110512268RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG289470.2578760340274852RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGC285040.25392954274776103No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA275760.24566240074418533RNA PCR Primer, Index 6 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT266960.237822869533898RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC264760.23586298673132616RNA PCR Primer, Index 5 (97% over 35bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG264550.23567590700926247RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG262240.23361803006656207RNA PCR Primer, Index 1 (100% over 29bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGGA259700.23135525628541095RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC257750.22961808743767687No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA248810.22165383641268038RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG247580.2205580837548789RNA PCR Primer, Index 1 (100% over 29bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA239620.21346687143284623RNA PCR Primer, Index 6 (100% over 38bp)
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG238420.21239784444962526No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT236230.21044687020524694No Hit
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA236110.21033996750692485RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA228100.2032042123939247No Hit
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA227700.20284787006618435RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC222960.19862521348246143RNA PCR Primer, Index 1 (100% over 33bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA199670.17787718144978054RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCC199530.17775246163507144No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC198090.17646962925520623No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCCA197240.17571240180875802RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG194750.17349417081857443RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA194430.17320909695638217No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC192160.1711868542464558RNA PCR Primer, Index 1 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG191590.17067906642942582RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC191300.1704207182418141RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG191040.17018909572878285RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG188810.1682024872516305RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA185160.16495086351099997RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG182120.16224266182017347RNA PCR Primer, Index 1 (100% over 29bp)
GGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA180530.16082620106740564RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG166750.14855020787675116RNA PCR Primer, Index 6 (100% over 34bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCC166420.1482562254563654No Hit
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTG161800.14414047157096455No Hit
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT161560.14392666617432034No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC158500.1412006473671068RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA152240.1356238899379706No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC148160.1319891981950192No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC148110.13194465540405168RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG148060.13190011261308413No Hit
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTGGAATTCTCGGGT148030.13187338693850362No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGC147020.13097362256095926No Hit
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA146240.13027875502186562RNA PCR Primer, Index 1 (100% over 28bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG146200.1302431207890916No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA145650.12975315008844862RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT143520.12785562719323135RNA PCR Primer, Index 6 (100% over 39bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA139700.12445255796331117RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTGGAATTCTCGGGTGCCAAGG139190.12399822149544225Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAACT138270.12317863414163949RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC136420.12153055087584044RNA PCR Primer, Index 1 (100% over 27bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA136240.12137019682835729No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA134520.11983792481907386No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG133400.11884016630140093No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG132100.11768205373624485RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT131990.11758405959611626No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA130570.11631904433263808RNA PCR Primer, Index 1 (100% over 28bp)
TGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCCAAGG126830.11298724356826598Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT125840.11210529630710864RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG123330.10986924820053806RNA PCR Primer, Index 6 (100% over 34bp)
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC120810.10762429153577398RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCTCGGGTGCCAAGGAA120600.10743721181371028RNA PCR Primer, Index 1 (100% over 23bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT119830.10675125283281015RNA PCR Primer, Index 1 (100% over 30bp)
AACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGGGTGCCAAGGAAC118670.1057178600823632RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA116790.10404305114198362RNA PCR Primer, Index 1 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAATGGAATTCTCGGGTGCCAAGGAACTCCA116190.10350853765037313RNA PCR Primer, Index 1 (100% over 28bp)
TCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTGTGGAATTCTCGG115200.10262659038921579No Hit
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC115080.10251968769089369RNA PCR Primer, Index 1 (100% over 27bp)
TGGTAGACTATGGAACGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT114790.10226133950328195RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACTC114750.10222570527050792RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)