FastQCFastQC Report
Fri 10 May 2024
EGAF00004834054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15580051
Total Bases794.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC8984485.766656347915678RNA PCR Primer, Index 7 (100% over 50bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3777522.4245876987180592RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC3314032.1270983002558848RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2933381.8827794594510634RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG2119461.3603678190783843No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1664791.068539506064518RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1348400.865465716383085No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1234940.7926418212623308RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1149330.7376933490140694RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC1034720.664131330507198No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1017180.6528733442528526RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA935290.6003125406970747RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT902730.579414021173615No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA856030.5494397932330259No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA683460.4386763560658435No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT676850.43443375121172584No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC643580.41307952072814136RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC615680.39517200553451337RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC584590.3752170002524382RNA PCR Primer, Index 1 (100% over 26bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT567980.36455593117121377No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA562730.36118623745198264RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA552980.3549282348305535RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG534740.34322095608031067No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC525760.3374571752043687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG524020.3363403624288521No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC502420.32247647969830134RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG493810.31695018199876235RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC491790.3156536522248868No Hit
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC458840.2945048125965698RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG454330.29161008523014464No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC419140.2690235096149557RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA399030.25611597805424385No Hit
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCA397520.2551467899559507RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA388600.24942151986537142No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG388120.2491134335824703No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA374600.24043566994742188RNA PCR Primer, Index 1 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA371610.23851654914351694RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC369310.23704030237128235RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA364520.2339658580064982RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA361150.23180283556196316RNA PCR Primer, Index 1 (100% over 32bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCC359700.2308721582490327RNA PCR Primer, Index 1 (100% over 27bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG353670.22700182432008725Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG343530.22049350159380093RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG337450.21659107534372No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA334510.2147040468609506RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC333710.21419056972278205RNA PCR Primer, Index 1 (100% over 31bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA311940.20021757310037047RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC311850.2001598069223265RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT306700.1968542978453665No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC305130.1958465989617107RNA PCR Primer, Index 1 (100% over 27bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA297690.19107126157674326No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC297530.19096856614910954RNA PCR Primer, Index 1 (100% over 27bp)
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG292900.18799681721195907No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC283310.18184151001816362RNA PCR Primer, Index 1 (100% over 26bp)
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGG283220.18178374384011964No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC282520.18133445134422216No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA279480.1793832382191817RNA PCR Primer, Index 1 (100% over 28bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGG278430.17870929947533548No Hit
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC275580.17688003717061004RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA264220.16958866180861668RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC263580.16917788009808182No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA262810.1686836583525946RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC254570.16339484382945857RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA252970.16236788955312148RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA248250.15933837443792706RNA PCR Primer, Index 7 (100% over 35bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC243750.156450065535729RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC239430.15367728898961885RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC236250.15163621736539887RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA233980.15017922598584563RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT232350.1491330163168272RNA PCR Primer, Index 1 (100% over 25bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC230150.1477209541868637No Hit
AATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTCC225540.14476204217816746RNA PCR Primer, Index 1 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCC225110.14448604821640187RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCTCGGGTGCCAAGGA223360.14336281697665815RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC222380.1427338074824017RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC219210.14069915432240884RNA PCR Primer, Index 1 (100% over 27bp)
TTGTGGGAACAATGGCACGGCAAGGGGCTCGGTATTTTTGGAATTCTCGG215230.1381446055600203No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG213460.1370085373918224No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC212430.1363474355764304RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA208590.13388274531322136RNA PCR Primer, Index 1 (100% over 28bp)
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTC205130.1316619566906424RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA203200.1304231930948108No Hit
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCA200000.1283692845421366RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG196760.126289702132554No Hit
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG191910.12317674698240717No Hit
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC185850.11928715766078044RNA PCR Primer, Index 1 (100% over 27bp)
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT184760.11858754506002579No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC184470.1184014095974397No Hit
TTGTGGGAACAATGGCACGGCAAGGGGCTCGGTATTTTTTGGAATTCTCG182640.11722683064387913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC179580.11526278059038446No Hit
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC177360.11383788153196675RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC175680.1127595795418128No Hit
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTC175440.11260553640036224RNA PCR Primer, Index 1 (100% over 26bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG174020.11169411448011307RNA PCR Primer, Index 7 (100% over 36bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGC173780.1115400713386625No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC173440.11132184355494086No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG171600.1101408461371532RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA166920.10713700487886722RNA PCR Primer, Index 1 (100% over 32bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA164990.1058982412830356RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA164220.10540401953754835No Hit
GGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCC162080.1040304681929475RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG159300.1022461351378118No Hit
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCT158020.10142457171674213No Hit
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAT156850.10067361140217064No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA156440.10041045436885926RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC156180.10024357429895447RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)