FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15580051
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC8983915.766290495454732RNA PCR Primer, Index 7 (100% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG3773792.4221936115613487RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC3299972.1180739395525725RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2930141.8806998770414807RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG2095131.3447516956138332No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1657881.0641043472835872RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1347700.8650164238871876No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1233770.7918908609477594RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1148000.7368396932718642RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC1031010.6617500802789413No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1016230.6522635901512774RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG934110.5995551619182762RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG901780.5788042670720398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA855340.5489969192013556No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA683050.438413199032532No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC675480.43355442161261215No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA643040.4127329236598776RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC613310.393650829512689RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC582370.3737921011940205RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG567570.3642927741379024No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG562140.36080754806258336RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG552460.35459447469074395RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC533670.3425341804080102No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA525480.33727745820600974No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGG518800.3329899241023023No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA502030.3222261595934442RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT493300.31662284032317994RNA PCR Primer, Index 1 (100% over 30bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT491650.3155637937257073No Hit
AATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA458360.29419672631366867RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGC453260.29092330955784423No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA418630.26869616793937323RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA398570.25582072869979694No Hit
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG397130.25489646985109354RNA PCR Primer, Index 1 (100% over 29bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC388270.24920971054587687No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC387350.24861921183698307No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG374110.24012116520029364RNA PCR Primer, Index 1 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG371290.2383111582882495RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA368790.2367065422314728RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG364240.23378614100813916RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC360760.231552515457106RNA PCR Primer, Index 1 (100% over 33bp)
TTAATGCTAATCGTGATAGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA359260.23058974582303998RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGGA353490.22688629196399934RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT343250.22031378459544193RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGT336890.216231641347002No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG334140.2144665636845476RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA333420.2140044342601959RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA311740.20008920381582831RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG311620.20001218224510306RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG306380.1966489069900991No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA304720.19558344192839935RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA297250.19078884915075053RNA PCR Primer, Index 1 (100% over 28bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA296980.19061555061661864No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGC292030.18743841082420076No Hit
GGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGG282820.18152700527103538No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC281920.18094934349059577RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCC281540.1807054418499657No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG279190.1791971027565956RNA PCR Primer, Index 1 (100% over 29bp)
ATTGGGGAGTGAGAGGGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGG277890.1783627024070717No Hit
CAAAGTGCTTACAGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA275300.17670032017225104RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC264000.16944745559562033RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC262610.16855528906805248No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG262470.16846543056887298RNA PCR Primer, Index 1 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATGGAATTCTCGGGTGCCAAGGAACTCC253660.16281076358479185RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG252660.16216891716208118RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG248240.15933195597369995RNA PCR Primer, Index 7 (100% over 36bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA243490.1562831854658242RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA239250.15356175663353092RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC236220.15161696197271757RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG233760.15003801977284925RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC231880.1488313484981532RNA PCR Primer, Index 1 (100% over 26bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG229660.14740644943973547No Hit
AATGGATTTTTGGAAATAGGAGATGGAATTCTCGGGTGCCAAGGAACTCCA225340.14463367289362533RNA PCR Primer, Index 1 (100% over 28bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA224920.14436409739608683RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCTCGGGTGCCAAGGAA223230.14327937694170578RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC221350.1420727056670097RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCA218950.14053227425250406RNA PCR Primer, Index 1 (100% over 28bp)
TTGTGGGAACAATGGCACGGCAAGGGGCTCGGTATTTTTGGAATTCTCGGG214950.13796488856166134No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG213280.13689300503573448No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC211520.13576335533176367RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG208420.13377363142136056RNA PCR Primer, Index 1 (100% over 29bp)
TTAATGCTAATCGTGATAGGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC204190.13105862105329436RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG202990.13028840534604155No Hit
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG199880.12829226297141133RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC196310.1260008712423342No Hit
GATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGT191560.12295210073445845No Hit
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA185640.1191523699120112RNA PCR Primer, Index 1 (100% over 28bp)
ATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTG184550.11845275731125655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC183910.11804197560072172No Hit
TTGTGGGAACAATGGCACGGCAAGGGGCTCGGTATTTTTTGGAATTCTCGG182550.11716906446583518No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA179460.11518575901965918No Hit
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA177230.11375444149701436RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTCG175550.11267613950686041No Hit
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC174650.11209847772642079RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA173950.11164918523052332RNA PCR Primer, Index 7 (100% over 37bp)
GAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATTCTCGGGTGCC173090.11109719730699212No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT172890.11096882802244998No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT171400.11001247685261106RNA PCR Primer, Index 1 (100% over 30bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC166800.10705998330814194RNA PCR Primer, Index 1 (100% over 33bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG164880.10582763817653743RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG164090.10532057950259598No Hit
GGAGAGAACGCGGTCTGAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA161970.10395986508644932RNA PCR Primer, Index 1 (100% over 28bp)
ACGTACTGGAGGTCCGAACCCACTAACGTTGAAAAGTTAGGGGATGAGCTG157620.10116783314765786No Hit
GTTAGGGGATGAGCTGTGGATAGGGGTGAAAGGCTAAACAAACTTGGAATT156590.10050673133226586No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG156270.10030134047699843RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA156030.10014729733554786RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1300300.044.8913651
TCCATAG456050.044.6829513
CCATAGG454950.044.66736214
TATTGCA418500.044.6328431
TATAGTT924600.044.6266116
CATAGGC457000.044.5754415
CAACGCT188750.044.5408067
TGTATAG938100.044.53328314
ATTCCAT519900.044.53072411
TTCCATA457750.044.52683312
ATAGTTT796750.044.51993617
TTGTATA941400.044.50144213
TTCAACG187050.044.496875
GTCTACG50450.044.46161
ATAGGCT588350.044.4395716
TAGTAGG790750.044.4332586
GTTGTAT741750.044.41443312
GTAGGTT791400.044.339918
TGTCCCG418050.044.31567410
TCAACGC189800.044.2588466
Produced by FastQC (version 0.11.5)