FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35051869
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT26050.035.1739851
CCGTCAT101600.023.2508891
ACCGTTG35200.022.6689363
GACCGTT38100.021.213112
GTATCGA60600.020.3792764
GACGCTA81350.018.97644
CGTATCG66650.018.528393
ACGCTAC84400.018.3463445
TACTCTA128700.017.936279
CCCGTAT73550.017.3243371
CGTCATC135100.016.9969962
CGCTACT96700.016.3074476
CCGTATC82150.015.7992132
CGGTAAT33750.014.56436351
AACCCGT65800.013.0054992
CTTGGAT234300.012.8320581
CAATCGG113500.012.7641534
TCATCTA191200.012.62013154
CGCGTAT6108.967618E-1012.5175171
CCGTTGA68700.012.3069754
Produced by FastQC (version 0.11.5)