FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35051869
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT27450.034.6120071
ACCGTTG36350.023.6522163
GACCGTT35800.023.3521732
CCGTCAT102600.023.2894821
GTATCGA68100.022.7389624
GACGCTA76500.020.5525974
CGTATCG77250.020.3529423
ACGCTAC80200.019.5451815
CCCGTAT84350.019.0357841
CGCTACT93700.017.033346
TACTCTA135550.016.9949079
CGTCATC140800.016.4969582
CGGTAAT32400.015.9816151
AACCCGT73150.014.93520452
CCGTATC112400.014.5797622
CCGTTGA69350.013.1508824
TTAAGGC154900.013.0327987
TAAGGCG157850.012.9991738
CTTGGAT238800.012.65208151
CAACCCG86700.012.6022441
Produced by FastQC (version 0.11.5)