FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31783163
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23050.014.1082741
CGTATCG32550.013.5612333
CCCGTAT46800.012.1600971
GTATCGA38350.011.8805214
CCGGTAT57350.011.2573531
GTCCGAT23000.010.8121351
GACGCTA39250.010.5198424
CCGGGAT80300.010.0648461
TCGACCG19700.09.8775047
CGGGGTT96200.09.7932561
CCGTATC54200.09.7328322
CGACCTA17150.09.6857564
CGCGAAT9952.1282176E-109.6126431
CGGTAAT30450.09.4232321
CGCGGAT32000.09.1162411
CCGGAAT68050.09.065621
CGCGAGT28500.09.0611811
ACGCTAC47250.09.0400395
CGGTATT56850.08.7775712
TATCGCG8751.0913936E-118.6784379
Produced by FastQC (version 0.11.5)