FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27687020
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16750.013.6178341
CGCGGGT51650.013.3407211
CGACCTA16250.012.8606724
CGCGTAT7201.0822987E-911.2201351
CCCGTAT35800.010.48638251
CGTATCG25600.010.3943363
CTCGGTT47750.09.5538741
CGCGGAT30700.09.4421981
GTATCGA27850.09.3799784
CCGGAAT60850.09.3713531
CGCGAGT27050.09.3108691
CGCGAAT10456.0754246E-109.0948461
CGCCAAT49200.09.0791331
CGGCGAT48600.08.6045471
CGAGAAT55850.08.5936931
CGGGGAT77500.08.5230441
CCGGTAT43500.08.5209281
CGACGAT22050.08.4049991
CGGGGTT90900.08.3121791
CGGAAAT59500.08.1463831
Produced by FastQC (version 0.11.5)