FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32933413
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22700.018.6365281
CCCGTAT43800.013.4570051
GTATCGA38850.012.22921854
CGTATCG35650.012.1274723
CGCGTAT7652.932211E-910.5630251
ACCGTTG34800.010.2393043
CGGGGTT99200.09.8229741
CGACCTA19700.09.4056354
CGCCAAT54750.09.2028541
CCGGGAT86700.09.1558411
GTCCGAT25200.09.0540221
CCCGGTT56900.08.9386591
CTCGGAT84250.08.80147651
CCGGTAT55050.08.7209721
CCCGGAT90450.08.5660351
CGGGAAT70850.08.5204931
CGGCAAT44750.08.4976291
CGCGAAT14601.8189894E-128.46489051
CGCCATT79950.08.3830391
CGGGGAT92600.08.3158161
Produced by FastQC (version 0.11.5)