FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32441551
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22500.018.3685471
GTATCGA38650.013.5190174
CCCGTAT48050.012.9513781
CGTATCG40200.012.879443
CCGGTAT64350.012.1069111
CGGTATT62850.010.4296652
CGCGTAA5556.7380297E-610.2713211
CGGCAAT42300.09.8828091
CGGTAAT30600.09.7804131
TCGACCG22400.09.5425937
ACCGTTG36500.09.3699263
CCGGGAT85250.09.1387661
GACGCTA40250.08.9691154
CGCCAAT54100.08.9565541
CTTGGAT217750.08.5301491
CCGCAAT40800.08.4996441
CGCCATT80350.08.4545061
CGGAAAT77650.08.3202331
CCGACAT61700.08.3152761
CGACCTA19450.08.3035014
Produced by FastQC (version 0.11.5)