FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30343781
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG40650.016.9414713
GTATCGA42650.016.7039324
CCCGTAT48950.015.821911
GACGCTA43700.014.7810064
ACGCTAC47900.013.7822265
CGACCGT21850.012.3950251
CCGTATC71450.010.8372712
CGCTACT63200.010.4464466
CCGGGAT86400.010.3937461
CGCGTAT7101.1897464E-710.0382251
CGCGGAT30400.09.3778151
CTTGGAT215500.09.3485151
CGAGAAT50650.09.2870921
CGGGGAT83950.09.282121
ATCGAAG103550.09.1279626
CGGGTAT28950.09.026891
CGGGGTT99350.08.9911241
CCGGGTT88250.08.8836871
CGCGAAT10203.783498E-98.8506971
TCGAAGG104950.08.8251397
Produced by FastQC (version 0.11.5)