FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33166216
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23650.016.0695461
CGTATCG35300.013.9946123
GTATCGA41300.013.1117194
CCCGTAT46150.012.6612961
CGACCTA19850.011.9650444
CCGGTAT57450.09.922841
CGGGGTT104050.09.6791791
CTCGGAT80750.09.6481941
ACCGTTG35750.09.4337533
CTCGGGT91750.08.8539041
CTCGGTT55500.08.7307581
CCGGGAT86450.08.6823411
CCCGGAT92700.08.5069351
GTTCGAT52750.08.4654521
ATCGAAG95900.08.3709146
CCCGGTT52800.08.3674621
CCGGAAT74800.08.319831
CGCGGTT33950.08.2557581
GACCGTT44500.08.2192642
CCGTCAT66150.08.1869051
Produced by FastQC (version 0.11.5)