FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32118593
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21200.015.29550651
CGTATCG35700.012.6395293
CCGGTAT61750.011.5064171
GTATCGA39450.011.4380544
CCCGTAT46500.011.3831081
CGGTATT59800.010.1746732
TATCGCG9950.010.0241159
GTCCGAT25550.09.7051731
CGCGTAT7402.0633706E-79.6660791
CGGTAAT29700.09.63353351
CCGGGAT84400.09.4919991
CCGTATC52850.09.3540572
CGCGTAA4600.00131491619.3298681
CCGCAAT35950.09.2851721
CGCGGAT32150.09.1960541
CCGACAT64800.09.0515081
CTCGGAT74450.09.0311951
CGGGGTT99300.08.8360751
TCGACCG22050.08.8313097
CGCGAGT30200.08.6845351
Produced by FastQC (version 0.11.5)