FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33099101
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20900.019.0071871
CGTATCG31550.011.2921273
CCCGTAT43750.011.0491621
CGCGTTT19150.010.9968921
CCGGTAT55800.010.3785621
CCGGGAT87250.010.2031341
CGCGTAA6101.7123639E-610.19998551
GTATCGA37300.010.1861414
ACCGTTG33100.010.0457853
CTCGGAT85050.09.00392151
CGCCATT86450.08.8581091
CCCGGAT88950.08.7705671
CGCAAAT37050.08.655111
CTCGGTT54450.08.6141851
CGGGTAT31700.08.4550241
CTCGGGT87550.08.4188081
CCGGCAT73800.08.3660291
CGGGGTT101950.08.1686091
CGACCTA18050.08.15667254
CGCCAAT55750.08.1557661
Produced by FastQC (version 0.11.5)