FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004834225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004834225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30591720
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTA43650.017.5193484
CGACCGT21900.015.9041921
ACGCTAC49100.015.6704765
CCCGTAT45900.013.097571
GTATCGA39200.012.7227084
CGTATCG38550.012.5677383
CGCTACT67050.011.6169926
CCGGTAT36650.011.3260411
CCGTATC57550.010.319152
CCGTCAT65700.010.3123071
CGCGTAT6503.6211532E-710.2765551
CTCGGTT53450.010.0870361
CGATAAC33850.09.7257541
TCGGATT44650.09.2571452
GTCCGAT25300.09.2407761
CTCGGAT76500.09.2306681
CAATCGG60000.09.1038054
CCGGGAT87400.08.8983411
ATTAGCG19750.08.8978136
GTCGGAT32400.08.8356631
Produced by FastQC (version 0.11.5)