FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42274
Sequences flagged as poor quality0
Sequence length50-251
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23355.523489615366419No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT20044.740502436485784No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19214.544164261721153No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12182.881203576666509No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9592.2685338505937453No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9542.25670624970431No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9122.157354402233051No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9062.143161281165728No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8542.020154231915598No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8381.9823059090694044No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7381.7457538912806925No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG7381.7457538912806925No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5761.3625396224629795No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5221.2348015328570752No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4341.026635757203009No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3850.9107252684865402No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG3720.8799735061740076No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3550.8397596631499267No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3490.8255665420826039No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3470.8208355017268296No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG3270.7735250981690873No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2910.6883663717651511No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2810.66471116998628No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2450.5795524435823438No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2420.5724558830486823No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2010.4754695557553106No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1970.4660074750437621No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1940.45891091451010074No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1820.43052467237545533No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1730.4092349907744713No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1650.3903108293513744No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.37611770828405167No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1550.3666556275725032No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1490.3524625065051805No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1400.33117282490419647No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1390.3288073047263093No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1360.32171074419264795No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1300.30751762312532527No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1200.28386242134645406No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1180.27913138099067986No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1130.2673037801012443No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1130.2673037801012443No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1110.26257273974547No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1090.2578416993896958No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1070.25311065903392155No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT1050.24837961867814734No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1040.2460140985002602No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA1020.24128305814448597No Hit
CCTACGGGAGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT1010.23891753796659887No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1000.2365520177887117No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1000.2365520177887117No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA990.23418649761082463No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA960.2270899370771633No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT950.22472441689927614No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC950.22472441689927614No Hit
CCTACGGGTGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT930.2199933765435019No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC900.21289681600984056No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT870.20580025547617922No Hit
CCTACGGGTGGCAGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT860.2034347352982921No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT850.201069215120405No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC850.201069215120405No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT850.201069215120405No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT840.19870369494251783No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC830.19633817476463075No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA830.19633817476463075No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA820.19397265458674362No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA810.19160713440885652No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC800.18924161423096938No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC790.18687609405308225No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC770.18214505369730805No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA760.17977953351942091No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT760.17977953351942091No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT750.1774140133415338No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT730.17268297298575958No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT730.17268297298575958No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA720.17031745280787244No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT720.17031745280787244No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA720.17031745280787244No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC720.17031745280787244No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA720.17031745280787244No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA710.16795193262998534No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA700.16558641245209824No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGGAACCCTGAT690.1632208922742111No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA670.15848985191843687No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC660.15612433174054974No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA650.15375881156266263No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT640.1513932913847755No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA630.1490277712068884No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT630.1490277712068884No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT630.1490277712068884No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA620.1466622510290013No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT620.1466622510290013No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA610.14429673085111416No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT600.14193121067322703No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT600.14193121067322703No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGGAACCCTGAT600.14193121067322703No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGAGGCAACTCTGAA600.14193121067322703No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT590.13956569049533993No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA580.1372001703174528No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGAGGCAACTCTGAA570.1348346501395657No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC560.1324691299616786No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA560.1324691299616786No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC560.1324691299616786No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA550.13010360978379146No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT550.13010360978379146No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA540.12773808960590435No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT540.12773808960590435No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA530.12537256942801722No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT520.1230070492501301No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT510.12064152907224299No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA510.12064152907224299No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA500.11827600889435586No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT500.11827600889435586No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT500.11827600889435586No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT490.11591048871646874No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT490.11591048871646874No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA480.11354496853858165No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC480.11354496853858165No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG480.11354496853858165No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT480.11354496853858165No Hit
CCTACGGGAGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC480.11354496853858165No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT480.11354496853858165No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA470.11117944836069452No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGAGGCAACTCTGAA470.11117944836069452No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA460.1088139281828074No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT460.1088139281828074No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC450.10644840800492028No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT450.10644840800492028No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT450.10644840800492028No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGGAACCCTGAT450.10644840800492028No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC440.10408288782703316No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT440.10408288782703316No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT430.10171736764914605No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGAGGCAACTCTGAA430.10171736764914605No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT430.10171736764914605No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT430.10171736764914605No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT430.10171736764914605No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT430.10171736764914605No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT430.10171736764914605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)