FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22901
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5692.48460765905419No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5062.2095105017248153No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4842.11344482773678No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3221.4060521374612462No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3121.3623859220121393No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3001.3099864634732108No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2971.2968865988384786No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2781.2139207894851753No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2771.2095541679402646No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2491.087288764682765No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG2411.0523557923234794No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2210.9650233614252653No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG2060.8995240382516048No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1980.8645910658923192No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1940.8471245797126763No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1940.8471245797126763No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1930.8427579581677657No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1810.7903584996288371No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1730.7554255272695516No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1610.7030260687306231No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1480.6462599886467839No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1440.6287935024671412No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1390.6069603947425877No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1340.5851272870180341No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1180.515261342299463No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1150.5021614776647307No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1060.46286188376053444No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1050.45849526221562376No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT930.4060958036766953No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC910.39736256058687397No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT820.3580629666826776No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC700.3056635081437492No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC670.29256364350901704No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC670.29256364350901704No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT660.2881970219641064No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC650.28383040041919566No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC630.2750971573293743No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT630.2750971573293743No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT620.27073053578446354No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC600.26199729269464217No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC600.26199729269464217No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC570.24889742805991005No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC560.2445308065149993No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC560.2445308065149993No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT550.24016418497008865No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC540.23579756342517796No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC510.22269769879044582No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC500.21833107724553513No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC500.21833107724553513No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC500.21833107724553513No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG480.20959783415571373No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC480.20959783415571373No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG470.20523121261080304No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC470.20523121261080304No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC460.20086459106589233No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC460.20086459106589233No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG450.19649796952098164No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT450.19649796952098164No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT450.19649796952098164No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC450.19649796952098164No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT440.1921313479760709No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC440.1921313479760709No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG440.1921313479760709No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC430.1877647264311602No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC430.1877647264311602No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC430.1877647264311602No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA410.1790314833413388No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT410.1790314833413388No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC410.1790314833413388No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC410.1790314833413388No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT400.1746648617964281No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA400.1746648617964281No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC400.1746648617964281No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT400.1746648617964281No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC380.1659316187066067No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT380.1659316187066067No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGAA380.1659316187066067No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT370.16156499716169598No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC370.16156499716169598No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC370.16156499716169598No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT360.1571983756167853No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT350.1528317540718746No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC350.1528317540718746No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT350.1528317540718746No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC340.1484651325269639No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT340.1484651325269639No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC340.1484651325269639No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC340.1484651325269639No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT330.1440985109820532No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT320.1397318894371425No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT320.1397318894371425No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC320.1397318894371425No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG320.1397318894371425No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT320.1397318894371425No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA300.13099864634732108No Hit
CCTACGGGAGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC300.13099864634732108No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT300.13099864634732108No Hit
CCTACGGGGGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC290.12663202480241037No Hit
CCTACGGGAGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC290.12663202480241037No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC290.12663202480241037No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT280.12226540325749966No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG270.11789878171258898No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT270.11789878171258898No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT270.11789878171258898No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT270.11789878171258898No Hit
CCTACGGGTGGCAGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT270.11789878171258898No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT270.11789878171258898No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC260.11353216016767827No Hit
CCTACGGGTGGCTGCAGTCACAGCTTCAGTGGACTGAATCTTTCCTGCCT260.11353216016767827No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC250.10916553862276757No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT250.10916553862276757No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT250.10916553862276757No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA250.10916553862276757No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA250.10916553862276757No Hit
CCTACGGGTGGCAGAGGCTGCAGTGAGCCGAGATTACACCACTGTACTCC240.10479891707785687No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC240.10479891707785687No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG230.10043229553294616No Hit
CCTACGGGTGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA230.10043229553294616No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC230.10043229553294616No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA230.10043229553294616No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT230.10043229553294616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)