FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59629
Sequences flagged as poor quality0
Sequence length147-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT53018.889969645642221No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT35946.027268610910799No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT35205.903167921648862No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT29064.873467608043066No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT27804.662161029029499No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT22493.7716547317580376No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19583.2836371564171793No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16062.693320364252293No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12352.0711398816012343No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11401.9118214291703701No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10781.807845176004964No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9931.6652970869878752No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9141.5328112160190512No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8611.4439282899260428No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8081.3550453638330342No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7901.324858709688239No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT6561.1001358399436516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6491.088396585554009No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6481.0867195492126316No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6431.0783343675057437No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6061.0162840228747756No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5560.9324322058058998No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4800.8049774438612085No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4680.7848530077646783No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4620.7747907897164131No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4160.6976471180130474No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3980.667460463868252No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3930.6590752821613645No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3750.6288886280165692No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3290.5517449563132033No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3180.5332975565580507No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3150.528266447533918No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3110.521558302168408No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2800.46957017558570496No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC2600.43602944875815464No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2510.42093612168575695No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2290.38404132217545156No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2210.3706250314444314No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC2040.3421154136410136No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1990.333730231934126No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1900.31863690486172835No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1710.28677321437555553No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC1380.23143101511009742No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC1330.22304583340320988No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT1310.2196917607204548No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1210.20292139730667963No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1170.19621325194116956No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1150.19285917925841453No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1050.17608881584463937No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC1030.17273474316188434No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC1020.1710577068205068No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC970.1626725251136192No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG940.15764141608948665No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC940.15764141608948665No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC930.15596437974810914No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT920.15428734340673164No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC880.14757919804122155No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC880.14757919804122155No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC870.14590216169984405No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC840.14087105267571148No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC830.13919401633433395No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT800.1341629073102014No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC770.12913179828606886No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC770.12913179828606886No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT760.12745476194469135No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC750.12577772560331382No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT740.12410068926193629No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT690.11571550755504871No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC670.11236143487229368No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC670.11236143487229368No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT670.11236143487229368No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT660.11068439853091616No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC640.10733032584816114No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG630.1056532895067836No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA610.10229921682402858No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT610.10229921682402858No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC610.10229921682402858No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC600.10062218048265106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)