FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44557
Sequences flagged as poor quality0
Sequence length35-251
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14063.15550867428238No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12092.7133783692797993No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11462.5719864443297347No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10102.2667594317391204No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9712.1792310972462237No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9592.1522993020176404No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9282.0827254976771328No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7981.79096438270081No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7981.79096438270081No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7821.755055322396032No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7801.7505666898579348No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6641.4902260026482932No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5301.1894876225957762No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5071.1378683484076577No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4881.0952263392957335No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4571.025652534955226No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4390.9852548421123505No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4100.92016967030994No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4020.902215140157551No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3990.895482191350405No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3930.8820162937361132No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3860.8663060798527729No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3780.8483515497003837No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3500.785510694167022No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3430.7698004802836815No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3370.7563345826693897No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3300.7406243687860493No Hit
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3150.7069596247503198No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2900.6508517180241039No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2810.6306528716026663No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2580.5790335974145476No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2460.552101802185964No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2460.552101802185964No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2350.527414323226429No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2140.4802836815764077No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2110.47355073276926185No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2010.4511075700787755No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1960.4398859887335323No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1920.4309087236573378No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1820.4084655609668515No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1700.38153376573826786No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1700.38153376573826786No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1650.37031218439302466No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1640.36806786812397607No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1640.36806786812397607No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1620.36357923558587874No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1590.35684628677873287No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1480.3321588078191979No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1470.32991449155014924No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1420.3186929102049061No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1370.3074713288596629No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1360.3052270125906143No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1340.300738380052517No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1340.300738380052517No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1160.2603406872096416No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1140.2558520546715443No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1120.25136342213344703No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1040.233408891981058No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1020.22892025944296068No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1000.22443162690486343No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA970.21769867809771754No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT960.2154543618286689No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG940.2109657292905716No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC940.2109657292905716No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG930.208721413021523No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG910.20423278048342575No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT900.20198846421437708No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG900.20198846421437708No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT880.19749983167627982No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG870.19525551540723118No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG860.19301119913818254No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG850.19076688286913393No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA830.18627825033103665No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT800.17954530152389073No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG800.17954530152389073No Hit
GACTACCAGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG790.17730098525484211No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC750.16832372017864758No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG750.16832372017864758No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG750.16832372017864758No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG750.16832372017864758No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA750.16832372017864758No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG750.16832372017864758No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA710.15934645510245304No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG700.1571021388334044No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG680.15261350629530715No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA670.1503691900262585No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG660.14812487375720987No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG650.14588055748816123No Hit
GACTACTAGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG650.14588055748816123No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT640.1436362412191126No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA610.1369032924119667No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA610.1369032924119667No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.1369032924119667No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA600.13465897614291805No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG600.13465897614291805No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG590.13241465987386944No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG590.13241465987386944No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA580.1301703436048208No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA580.1301703436048208No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG560.12568171106672352No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA550.12343739479767489No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG540.12119307852862626No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG530.11894876225957762No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG530.11894876225957762No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT530.11894876225957762No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG510.11446012972148034No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG510.11446012972148034No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA510.11446012972148034No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG510.11446012972148034No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG500.11221581345243171No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA500.11221581345243171No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA500.11221581345243171No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG500.11221581345243171No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG490.10997149718338307No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG490.10997149718338307No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA490.10997149718338307No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG490.10997149718338307No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG490.10997149718338307No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG490.10997149718338307No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG490.10997149718338307No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT480.10772718091433445No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG470.1054828646452858No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA450.10099423210718854No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA450.10099423210718854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)