FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45908
Sequences flagged as poor quality0
Sequence length50-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT23465.110220440881764No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT22494.898928291365339No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT19224.186634137840899No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13973.0430426069530365No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12302.6792715866515637No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11132.4244140454822687No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10922.3786703842467545No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10742.3394615317591705No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10722.3351049925938834No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10532.293717870523656No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10482.282826522610438No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10432.2719351746972203No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9462.0606430251807963No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT8831.9234120414742528No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8531.8580639539949464No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8361.821033371090006No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8011.744793935697482No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7991.7404373965321949No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7741.6859806569661062No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6901.503006012024048No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6731.4659754291191076No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6621.4420144637100287No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6531.422410037466237No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6411.3962708024745143No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5941.2938921320902674No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5781.2590398187679708No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT5131.1174522958961401No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5041.0978478696523482No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4851.0564607475821208No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4701.0237867038424675No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4661.0150736255118933No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4470.973686503441666No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4360.949725538032587No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4190.9126949551276466No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3990.8691295634747755No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3550.7732857018384596No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3500.7623943539252418No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3470.7558595451773111No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3200.6970462664459354No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2940.640411257297203No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2900.6316981789666289No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2900.6316981789666289No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2720.5924893264790451No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT2660.5794197089831837No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2660.5794197089831837No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA2640.5750631698178967No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2380.5184281606691644No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT2340.5097150823385902No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT2010.4378321861113531No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1940.4225842990328483No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1890.41169295111963056No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1760.38337544654526445No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA1430.31149255031802736No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1400.3049577415700967No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA1390.3027794719874532No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1370.2984229328221661No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1310.28535331532630476No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1300.28317504574366126No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA1270.2766402369957306No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1250.2722836978304435No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1110.24178792367343382No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1070.23307484534285963No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1070.23307484534285963No Hit
CCTACGGGCGGCAGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA1060.23089657576021608No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT960.2091138799337806No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA940.2047573407684935No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT910.19822253202056286No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT890.19386599285527575No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT880.19168772327263223No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT740.16119194911562257No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT720.15683540995033546No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGATGCAAGTCTGAC670.1459440620371177No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG640.13940925328918705No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT610.1328744445412564No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT590.12851790537596933No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC570.12416136621068224No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT560.12198309662803869No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA560.12198309662803869No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC550.11980482704539513No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT520.1132700182974645No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT500.1089134791321774No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT480.1045569399668903No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC470.10237867038424675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)