FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45908
Sequences flagged as poor quality0
Sequence length50-251
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG19084.15613836368389No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16653.6268188551015075No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14973.260869565217391No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14803.223838982312451No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13312.8992768144985623No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12592.742441404548227No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11672.5420406029450207No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11552.515901367953298No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11372.476692515465714No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10852.3634224971682496No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10422.2697569051145767No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10292.241439400540211No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9081.9778687810403415No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8411.8319247190032237No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8311.8101420231767882No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7721.681624117800819No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7521.638058726147948No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7381.6075629519909385No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6961.5160756295199094No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6311.3744881066480787No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6141.3374575237431385No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6061.32003136708199No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5761.2546832796026837No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5681.2372571229415352No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5481.1936917312886643No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5021.093491330487061No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4601.002004008016032No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4590.9998257384333886No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4150.9039818767970723No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4100.8930905288838547No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3840.8364555197351224No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG3740.8146728239086869No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3650.7950683976648949No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3560.775463971421103No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG3340.727542040602945No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3010.6556591443757079No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG2910.6338764485492724No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2790.6077372135575498No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2740.5968458656443322No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG2540.5532804739914612No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2200.47921930818158054No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG2150.4683279602683628No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2150.4683279602683628No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2040.44436699485928377No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1970.42911910778077894No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1970.42911910778077894No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1880.409514681536987No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1840.4008016032064128No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1830.3986233336237693No Hit
GACTACCAGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1620.35287967238825474No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1590.34634486364032413No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1530.3332752461444628No Hit
GACTACTAGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1450.31584908948331447No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1450.31584908948331447No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1370.2984229328221661No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1350.294066393656879No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1320.28753158490894837No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1240.27010542824779993No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1190.2592140803345822No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1190.2592140803345822No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1130.2461444628387209No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1090.23743138450814674No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1070.23307484534285963No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG1030.22436176701228547No Hit
GACTACACGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG1020.22218349742964189No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1010.22000522784699833No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG1010.22000522784699833No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.21347041909906772No Hit
GACTACCCGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG950.20693561035113706No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA900.1960442624379193No Hit
GACTACTCGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG890.19386599285527575No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG880.19168772327263223No Hit
GACTACAAGGGTATCTAAGCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG880.19168772327263223No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA820.17861810577677095No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG740.16119194911562257No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG690.15030060120240482No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG660.14376579245447418No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG650.14158752287183063No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG630.13723098370654352No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG630.13723098370654352No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG620.13505271412389996No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG620.13505271412389996No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA620.13505271412389996No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA590.12851790537596933No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG580.1263396357933258No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA580.1263396357933258No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA570.12416136621068224No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG550.11980482704539513No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG550.11980482704539513No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA520.1132700182974645No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG510.11109174871482094No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG500.1089134791321774No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG490.10673520954953386No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG470.10237867038424675No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT470.10237867038424675No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG470.10237867038424675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)