FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62997
Sequences flagged as poor quality0
Sequence length49-251
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC14182.250900836547772No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC11361.8032604727209232No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC10901.7302411225931391No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC9511.5095956950330967No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC8751.3889550296045843No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8651.3730812578376748No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC7991.268314364176072No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6651.055605822499484No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6441.022270901788974No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6371.0111592615521374No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6070.9635379462514088No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5650.8968681048303888No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5560.8825817102401702No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5490.8714700700033335No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5220.8286108862326778No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5180.822261377525914No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5020.7968633426988586No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4620.7333682556312205No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4530.7190818610410019No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4440.7047954664507834No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4350.6905090718605648No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4280.6793974316237281No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4180.6635236598568186No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4120.6539993967966728No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4010.6365382478530723No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3780.6000285727891804No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3550.5635188977252885No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3530.5603441433719065No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3510.5571693890185246No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3510.5571693890185246No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3410.5412956172516151No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3370.5349461085448514No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3360.5333587313681604No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3320.5270092226613966No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3320.5270092226613966No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3140.4984364334809594No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3010.47780053018397706No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2960.4698636443005223No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2930.4651015127704494No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2880.45716462688699455No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2820.44764036382684885No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2790.44287823229677603No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2740.4349413464133213No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2600.41271806593964794No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2540.4031938028795022No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2530.4016064257028112No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2510.3984316713494293No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2480.39366953981935643No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2410.3825578995825198No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2400.38097052240582885No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2390.3793831452291379No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2370.37620839087575597No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2370.37620839087575597No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2290.36350937346222834No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2110.3349365842817912No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2050.3254123212216455No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG2010.31906281251488167No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2010.31906281251488167No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1980.3143006809848088No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1970.31271330380811785No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1950.3095385494547359No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1820.2889026461577536No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1820.2889026461577536No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1820.2889026461577536No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1780.2825531374509897No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1770.28096576027429876No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1720.273028874390844No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1700.2698541200374621No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1690.2682667428607711No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1670.26509198850738924No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1640.2603298569773164No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG1590.2523929710938616No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1570.2492182167404797No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1570.2492182167404797No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.2492182167404797No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1540.24445608521040685No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG1530.24286870803371588No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1510.239693953680334No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.23810657650364303No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1480.23493182215026115No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG1470.23334444497357018No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1460.23175706779687924No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1450.23016969062018827No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1440.22858231344349728No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1440.22858231344349728No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1440.22858231344349728No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1430.22699493626680634No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1430.22699493626680634No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1410.22382018191342443No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1390.22064542756004255No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1380.21905805038335158No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1360.2158832960299697No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1340.2127085416765878No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1330.21112116449989682No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1300.20635903296982397No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1250.19842214708636918No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1250.19842214708636918No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1240.19683476990967821No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1240.19683476990967821No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1230.19524739273298727No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1220.19366001555629633No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG1200.19048526120291442No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG1190.18889788402622348No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG1170.18572312967284157No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG1140.1809609981427687No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG1090.17302411225931394No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1090.17302411225931394No Hit
GACTACACGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1090.17302411225931394No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.16984935790593203No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG1050.16667460355255012No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1040.16508722637585915No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1030.1634998491991682No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1030.1634998491991682No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1030.1634998491991682No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1020.16191247202247727No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT1020.16191247202247727No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA990.1571503404924044No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG990.1571503404924044No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTG980.15556296331571345No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG980.15556296331571345No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG970.15397558613902249No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG960.15238820896233155No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG940.14921345460894964No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG920.14603870025556773No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCAG900.14286394590218582No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTG880.1396891915488039No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG880.1396891915488039No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG870.13810181437211294No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT870.13810181437211294No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG850.13492706001873106No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT840.1333396828420401No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG830.13175230566534915No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG820.1301649284886582No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTG810.12857755131196724No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT810.12857755131196724No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG780.12381541978189438No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG780.12381541978189438No Hit
GACTACAAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT770.12222804260520342No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG770.12222804260520342No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG770.12222804260520342No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG770.12222804260520342No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCAG770.12222804260520342No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG760.12064066542851247No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG760.12064066542851247No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT750.11905328825182152No Hit
GACTACCAGGGTATCTAATCCTGGACGGCTGTCTGACATCACCCCGTTTC730.11587853389843962No Hit
GACTACCGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG730.11587853389843962No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGAG730.11587853389843962No Hit
GACTACCCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT710.1127037795450577No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTG710.1127037795450577No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT710.1127037795450577No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT700.11111640236836674No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG700.11111640236836674No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG690.10952902519167579No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG690.10952902519167579No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA670.1063542708382939No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA660.10476689366160294No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA660.10476689366160294No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG650.10317951648491198No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG650.10317951648491198No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCAG640.10159213930822103No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG640.10159213930822103No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG640.10159213930822103No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG640.10159213930822103No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG640.10159213930822103No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA640.10159213930822103No Hit
GACTACCAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAG630.10000476213153006No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCTTCAG630.10000476213153006No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT630.10000476213153006No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.10000476213153006No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG630.10000476213153006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)