FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26389
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG10233.8766152563568155No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC6912.618515290461935No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5842.213043313501838No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5472.0728333775436734No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4731.792413505627345No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4331.6408351964833832No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4311.6332562810261853No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3421.2959945431808708No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3371.2770472545378755No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT3161.1974686422372958No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2981.1292584031225132No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2651.004206298078745No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2550.9663117207927545No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2520.9549433476069574No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1920.7275758838910151No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1660.6290499829474402No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1430.5418924551896623No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1270.4812611315320778No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1240.46989275834628064No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC1240.46989275834628064No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1230.4661033006176816No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1040.3941036037742999No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT1010.3827352305885028No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC850.32210390693091817No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC810.306946076016522No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC780.29557770283072493No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT770.29178824510212586No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA660.25010421008753647No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG660.25010421008753647No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG650.24631475235893743No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG640.2425252946303384No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC640.2425252946303384No Hit
CCTACGGGTGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG630.23873583690173936No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG620.23494637917314032No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC610.23115692144454128No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC610.23115692144454128No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC600.22736746371594224No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT580.2197885482587442No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC560.21220963280154612No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT550.20842017507294708No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC550.20842017507294708No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC530.200841259615749No Hit
CCTACGGGAGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC520.19705180188714996No Hit
CCTACGGGTGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA520.19705180188714996No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC520.19705180188714996No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA500.18947288642995186No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG480.18189397097275378No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC470.17810451324415474No Hit
CCTACGGGAGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC470.17810451324415474No Hit
CCTACGGGGGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC460.17431505551555573No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG450.1705255977869567No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG450.1705255977869567No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC450.1705255977869567No Hit
CCTACGGGCGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC450.1705255977869567No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA440.16673614005835766No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC440.16673614005835766No Hit
CCTACGGGAGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT430.16294668232975862No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT430.16294668232975862No Hit
CCTACGGGGGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA420.15915722460115958No Hit
CCTACGGGTGGCAGCGAAGGCCATCCCACCGGTGGCCCAGGCGGCTGCTA420.15915722460115958No Hit
CCTACGGGAGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG410.15536776687256054No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC410.15536776687256054No Hit
CCTACGGGTGGCAGCAGGGGTTGGGAGGAGCAGCAGGGGTTGGGGGGAGC400.1515783091439615No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT400.1515783091439615No Hit
CCTACGGGAGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA400.1515783091439615No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC400.1515783091439615No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT390.14778885141536247No Hit
CCTACGGGGGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA380.14399939368676343No Hit
CCTACGGGTGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC380.14399939368676343No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT370.1402099359581644No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT360.13642047822956535No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCCACTCC360.13642047822956535No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT360.13642047822956535No Hit
CCTACGGGGGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC340.12884156277236727No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT340.12884156277236727No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT330.12505210504376824No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT320.1212626473151692No Hit
CCTACGGGTGGCAGGCGGGCGGACGCTCCCTACAAAGCCTTCAGGAGCAT310.11747318958657016No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG310.11747318958657016No Hit
CCTACGGGTGGCTGAGGCAGGAGGATCGCTTGAACCCGGGAGGTGGAGGT310.11747318958657016No Hit
CCTACGGGTGGCAGGAGCTTAGTGTGCCTAGAGGAAGATGTGTCTACAAA310.11747318958657016No Hit
CCTACGGGAGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC310.11747318958657016No Hit
CCTACGGGTGGCTGCAATCATAGAACACGGCAATTTTTCTAGGTGACGGG300.11368373185797112No Hit
CCTACGGGAGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC300.11368373185797112No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT300.11368373185797112No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT300.11368373185797112No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT300.11368373185797112No Hit
CCTACGGGGGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC300.11368373185797112No Hit
CCTACGGGGGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT300.11368373185797112No Hit
CCTACGGGTGGCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAAT300.11368373185797112No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG290.1098942741293721No Hit
CCTACGGGGGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG290.1098942741293721No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC290.1098942741293721No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT290.1098942741293721No Hit
CCTACGGGTGGCTGCTCCACAATGTTTCCCTTTAGTCGGGAGGGGAAATG280.10610481640077306No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT280.10610481640077306No Hit
CCTACGGGGGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC280.10610481640077306No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCCACTCC280.10610481640077306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)