FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41857
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC17524.185679814606876No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG14323.4211720859115555No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC11092.649497097259718No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10782.5754354110423585No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9702.317414052607688No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9492.2672432329120578No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG9062.164512506868624No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6991.6699715698688393No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC6731.6078553169123444No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG6621.5815753637384429No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6511.5552954105645411No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5741.3713357383472298No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5731.368946651695057No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4801.1467615930429795No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3920.9365219676517668No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3630.8672384547387535No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT3230.7716749886518384No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2590.6187734429127744No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2390.5709917098693169No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2310.551879016651934No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT2250.5375444967388967No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2110.5040972836084765No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1810.43242468404329026No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1740.4157010774780801No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC1680.40136655756504286No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1610.38464295099983276No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG1590.37986477769548704No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1550.3703084310867955No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1530.36553025778244974No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC1520.3631411711302769No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA1500.3583629978259311No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1430.341639391260721No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1390.33208304465202954No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1330.3177485247389923No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1240.2962467448694364No Hit
CCTACGGGAGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC1220.29146857156509065No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT1190.2843013116085721No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1160.2771340516520534No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC1150.27474496499988055No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1140.2723558783477077No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1130.2699667916955348No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1130.2699667916955348No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1120.2675777050433619No Hit
CCTACGGGAGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC1070.25563227178249753No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1070.25563227178249753No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC980.2341304919129417No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT960.22935231860859592No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT940.22457414530425018No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG890.21262871204338585No Hit
CCTACGGGAGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC890.21262871204338585No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC850.20307236543469434No Hit
CCTACGGGTGGCAGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT840.20068327878252143No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG820.19590510547817572No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT800.19112693217382992No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC750.17918149891296556No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT730.17440332560861985No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA720.17201423895644694No Hit
CCTACGGGTGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG720.17201423895644694No Hit
CCTACGGGGGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA720.17201423895644694No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT690.16484697899992834No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC680.16245789234775546No Hit
CCTACGGGTGGCTGCAGTCACAGCTTCAGTGGACTGAATCTTTCCTGCCT680.16245789234775546No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC670.16006880569558257No Hit
CCTACGGGTGGCTGCTCTCCAGCGCATACCCTCCTGAGTGAGAGCCCTGT660.15767971904340972No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCCACTCC650.15529063239123683No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC650.15529063239123683No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC640.15290154573906395No Hit
CCTACGGGGGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC630.1505124590868911No Hit
CCTACGGGTGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA600.14334519913037244No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG580.1385670258260267No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG570.13617793917385385No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA560.13378885252168096No Hit
CCTACGGGAGGCTGCTCTCCAGCGCATACCCTCCTGAGTGAGAGCCCTGT550.13139976586950808No Hit
CCTACGGGGGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC550.13139976586950808No Hit
CCTACGGGAGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT530.12662159256516234No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG530.12662159256516234No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC520.12423250591298947No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC520.12423250591298947No Hit
CCTACGGGTGGCAGCGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC520.12423250591298947No Hit
CCTACGGGCGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC520.12423250591298947No Hit
CCTACGGGGGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT510.1218434192608166No Hit
CCTACGGGCGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC490.11706524595647085No Hit
CCTACGGGTGGCAGCTTTGAAACGATTACTTAGCTTAAGGGGATGGGGGA480.11467615930429796No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC470.11228707265212509No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC460.10989798599995222No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC440.10511981269560647No Hit
CCTACGGGTGGCAGAGGCTGCAGTGAGCCGAGATTACACCACTGTACTCC440.10511981269560647No Hit
CCTACGGGCGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA440.10511981269560647No Hit
CCTACGGGGGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC420.10034163939126071No Hit
CCTACGGGGGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC420.10034163939126071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)