FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50929
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC19343.7974434997741953No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC14242.7960494021088182No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC13632.6762748139566845No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC13202.5918435468986236No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC11502.2580455143434977No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC10322.0263504093934697No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7431.4588937540497555No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG7001.3744624869916944No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC6411.2586149345166802No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6281.2330892026154057No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT5581.0956429539162362No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5401.0602996328221643No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5221.024956311728092No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG5181.0171022403738537No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5171.0151387225352941No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5080.9974670619882583No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG5070.9955035441496985No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG4880.9581967052170669No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4490.8816195095132439No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4380.8600208132890887No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG4330.8502032240962909No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4050.7952247246166231No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT3970.7795165819081467No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT3890.7638084391996701No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT3580.7029393862043236No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG3540.6950853148500854No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3520.6911582791729663No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT3520.6911582791729663No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3280.6440338510475369No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3250.6381432975318581No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3130.6145810834691433No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3090.6067270121149051No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3050.5988729407606668No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3030.5949459050835477No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2890.5674566553437138No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2820.5537120304737969No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2780.5458579591195586No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2640.5183687093797247No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2590.5085511201869268No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2530.49677001315556957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2310.4535726207072591No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG2230.43786447799878264No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2230.43786447799878264No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2140.4201928174517465No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2130.41822929961318694No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2120.4162657817746275No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2090.4103752282589487No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2050.40252115690471046No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2010.3946670855504722No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1980.3887765320347935No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1930.3789589428419957No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG1900.37306838932631703No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1880.3691413536491979No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG1870.36717783581063834No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1820.35736024661784055No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1790.3514696931021618No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1740.341652103909364No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1730.33968858607080443No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1710.3357615503936853No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1700.3337980325551258No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1660.32594396120088753No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1570.3082723006538514No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1540.30238174713817273No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1530.3004182292996132No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1520.2984547114610536No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1510.29649119362249404No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1510.29649119362249404No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1440.2827465687525771No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1430.28078305091401756No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1380.2709654617212197No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1370.2690019438826602No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1360.2670384260441006No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1320.25918435468986234No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1310.2572208368513028No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1300.25525731901274323No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1280.2513302833356241No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1260.24740324765850497No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1260.24740324765850497No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1220.23954917630426673No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1200.2356221406271476No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1170.2297315871114689No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1160.22776806927290935No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1140.22384103359579022No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.21205992656443284No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1070.21009640872587326No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1060.20813289088731374No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1050.20616937304875413No Hit
GACTACCGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1020.20027881953307547No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1010.1983153016945159No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG990.19438826601739675No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG980.1924247481788372No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG960.18849771250171807No Hit
GACTACACGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG960.18849771250171807No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG940.18457067682459896No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT940.18457067682459896No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT910.17868012330892027No Hit
GACTACCGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT900.1767166054703607No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG890.17475308763180114No Hit
GACTACCAGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC890.17475308763180114No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.1668990162775629No Hit
GACTACTAGGGTATCTAATCCTGGACGGCTGTCTGACATCACCCCGTTTC750.14726383789196723No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG720.14137328437628854No Hit
GACTACCCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT720.14137328437628854No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA690.13548273086060986No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.13548273086060986No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG680.1335192130220503No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG680.1335192130220503No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG660.12959217734493117No Hit
GACTACCAGGGTATCTAATCCTGGACGGCTGTCTGACATCACCCCGTTTC650.12762865950637162No Hit
GACTACTAGGGTATCTAATCCTTGGCACTGTTACCATTTTGGGTCAGATA640.12566514166781206No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG640.12566514166781206No Hit
GACTACTCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT630.12370162382925248No Hit
GACTACCAGGGTATCTAATCCCAGTTTGCATCTTCGCTATTGTGTATTCA620.12173810599069293No Hit
GACTACTAGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC620.12173810599069293No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT620.12173810599069293No Hit
GACTACCAGGGTATCTAATCCTTGGCACTGTTACCATTTTGGGTCAGATA610.11977458815213336No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG610.11977458815213336No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG600.1178110703135738No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG590.11584755247501424No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG590.11584755247501424No Hit
GACTACAAGGGTATCTAATCCTGGACGGCTGTCTGACATCACCCCGTTTC580.11388403463645468No Hit
GACTACAAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT580.11388403463645468No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG570.11192051679789511No Hit
GACTACTGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG560.10995699895933556No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG560.10995699895933556No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG550.10799348112077599No Hit
GACTACAGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT540.10602996328221642No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG540.10602996328221642No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG540.10602996328221642No Hit
GACTACAAGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC530.10406644544365687No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG510.10013940976653773No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG510.10013940976653773No Hit
GACTACACGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC510.10013940976653773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)