FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44369
Sequences flagged as poor quality0
Sequence length42-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG23525.30099844486015No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC21184.773603191417431No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG17764.002794744078073No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC16743.772904505397913No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG12962.9209583267596746No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12512.8195361626360746No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG12412.7969979039419415No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10342.3304559489733823No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8341.8796907750907164No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5381.212558317744371No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5171.1652279744866911No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4941.1133899794901847No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4410.9939372084112781No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4090.9218147805900516No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3740.8429308751605851No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3720.8384232234217585No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3510.7910928801640784No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3420.7708084473393585No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3280.7392548851675719No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3060.6896707160404787No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3060.6896707160404787No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3010.6784015866934121No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2790.6288174175663188No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2780.6265635916969055No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2750.6198021140886656No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2630.5927562036557056No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2560.5769794225698123No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2560.5769794225698123No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2480.5589488156145056No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2420.5454258603980257No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2150.4845725619238657No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2140.4823187360544524No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2100.47330343257679913No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2090.47104960670738577No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2060.4642881290991458No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1870.42146543758029253No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1860.41921161171087923No Hit
CCTACGGGAGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1810.40794248236381253No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1800.4056886564943993No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1790.40343483062498586No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1650.3718812684531993No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1650.3718812684531993No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1560.3515968356284793No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1490.335820054542586No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1490.335820054542586No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1470.3313124028037594No Hit
CCTACGGGCGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1460.32905857693434604No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1430.32229709932610606No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1410.3177894475872794No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1380.3110279699790394No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1340.3020126665013861No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC1320.2975050147625594No Hit
CCTACGGGGGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1250.28172823367666616No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG1210.2727129301990128No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1210.2727129301990128No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1160.2614438008519462No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1120.25242849737429285No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1120.25242849737429285No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1110.25017467150487954No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1110.25017467150487954No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1080.24341319389663954No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1060.2389055421578129No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1060.2389055421578129No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1040.23439789041898623No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1030.2321440645495729No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC1020.22989023868015956No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG1020.22989023868015956No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC990.2231287610719196No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT980.22087493520250626No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC980.22087493520250626No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT960.21636728346367962No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT940.21185963172485295No Hit
CCTACGGGAGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC920.20735197998602628No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC890.2005905023777863No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT870.19608285063895964No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC860.1938290247695463No Hit
CCTACGGGAGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT820.18481372129189297No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT820.18481372129189297No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC790.178052243683653No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT790.178052243683653No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT790.178052243683653No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC780.17579841781423966No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG780.17579841781423966No Hit
CCTACGGGGGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC770.17354459194482633No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT750.1690369402059997No Hit
CCTACGGGTGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT750.1690369402059997No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT730.16452928846717302No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG720.16227546259775968No Hit
CCTACGGGTGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC710.16002163672834635No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT690.1555139849895197No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC690.1555139849895197No Hit
CCTACGGGCGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC690.1555139849895197No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG640.14424485564245307No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT620.1397372039036264No Hit
CCTACGGGCGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG610.13748337803421307No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT600.13522955216479976No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC590.13297572629538643No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG590.13297572629538643No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC590.13297572629538643No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC580.1307219004259731No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC580.1307219004259731No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT570.12846807455655976No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC550.1239604228177331No Hit
CCTACGGGGGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC550.1239604228177331No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC530.11945277107890645No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT520.11719894520949312No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT520.11719894520949312No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT500.11269129347066646No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTACGCAATGGGGGAAACCCTGAC480.10818364173183981No Hit
CCTACGGGCGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC470.10592981586242647No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT470.10592981586242647No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC460.10367598999301314No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTAAGTCGGGAGGGGAAATG460.10367598999301314No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA460.10367598999301314No Hit
CCTACGGGTGGCTGCGAAGGCCATCCCACCGGTGGCCCAGGCGGCTGCTA460.10367598999301314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)