FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50552
Sequences flagged as poor quality0
Sequence length39-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG12642.500395632220288No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG9601.8990346573824972No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7691.5212058870074379No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6901.3649311599936698No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA6511.287782877037506No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6451.2759139104288653No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5751.1374426333280583No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5741.135464472226618No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5651.1176610223136572No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5401.0682069947776547No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5381.0642506725747745No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5291.0464472226618136No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5161.0207311283430922No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC5101.0088621617344518No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA5050.9989713562272512No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4690.9277575565754075No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4650.9198449121696471No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4540.8980851400538059No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4480.8862161734451655No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4340.858521918025004No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4290.8486311125178034No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4220.8347839848077228No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4130.8169805348947617No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4030.7971989238803607No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC3990.7892862794746004No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3840.7596138629529988No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3630.7180724798227568No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3560.7042253521126761No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3540.7002690299097959No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3450.6824655799968349No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3350.6626839689824339No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3310.6547713245766735No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3090.6112517803449913No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3050.6033391359392309No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3020.5974046526349106No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2990.5914701693305903No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2960.5855356860262699No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2860.5657540750118689No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2770.547950625098908No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2760.5459724639974679No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC2720.5380598195917076No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2680.5301471751859471No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2610.5163000474758664No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2520.4984965975629055No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2480.49058395315714515No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2460.4866276309542649No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2430.48069314764994464No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2420.4787149865485045No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2420.4787149865485045No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2400.4747586643456243No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2370.46882418104130397No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2360.4668460199398639No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2310.4569552144326634No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2270.44904257002690295No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2240.44310808672258273No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2140.4233264757081817No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2130.42134831460674155No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2130.42134831460674155No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2060.40750118689666087No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2040.4035448646937807No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2030.40156670359234053No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT2020.3995885424909005No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2010.39761038138946037No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2000.3956322202880202No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1930.3817850925779396No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1890.37387244817217913No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1880.37189428707073907No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1750.3461781927520177No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1740.3442000316505776No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1740.3442000316505776No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1710.3382655483462573No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1690.3343092261433771No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1680.332331065041937No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1650.3263965817376167No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC1610.3184839373318563No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1590.3145276151289761No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1560.3085931318246558No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1540.3046368096217756No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC1530.30265864852033547No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1530.30265864852033547No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1510.2987023263174553No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1480.29276784301313497No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1440.2848551986073746No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1420.2808988764044944No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1390.2749643931001741No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1390.2749643931001741No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1340.26507358759297356No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1330.2630954264915335No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1250.24727013768001266No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1240.24529197657857257No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1160.22946668776705173No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1130.22353220446273145No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1120.22155404336129136No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1090.21561956005697103No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC1080.21364139895553094No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1070.21166323785409086No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1050.20770691565121063No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC1050.20770691565121063No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1040.20572875454977052No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1020.20177243234689035No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT990.19583794904257001No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT990.19583794904257001No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT960.1899034657382497No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG950.18792530463680962No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC940.18594714353536954No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT920.1819908213324893No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG900.17803449912960911No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT890.17605633802816903No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC870.1721000158252888No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT860.17012185472384872No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTACGCAATGGGGGAAACCCTGAC840.1661655325209685No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG830.1641873714195284No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT820.1622092103180883No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG810.1602310492166482No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA800.1582528881152081No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT800.1582528881152081No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG760.1503402437094477No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT750.1483620826080076No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC740.14638392150656748No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT730.1444057604051274No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC710.1404494382022472No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC690.13649311599936698No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT680.1345149548979269No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC670.13253679379648678No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC650.12858047159360658No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT640.1266023104921665No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG640.1266023104921665No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA640.1266023104921665No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC630.12462414939072637No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC610.12066782718784616No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC600.11868966608640608No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT600.11868966608640608No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC590.11671150498496598No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGAC590.11671150498496598No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA580.11473334388352587No Hit
CCTACGGGAGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG570.11275518278208577No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC560.11077702168064568No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTACGCAATGGGGGAAACCCTGAC550.10879886057920557No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT550.10879886057920557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT520.10286437727488526No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTACGCAATGGGGGAAACCCTGAC510.10088621617344518No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC510.10088621617344518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)