FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34601
Sequences flagged as poor quality0
Sequence length35-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG10222.953671859194821No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC6561.8958989624577323No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6501.8785584231669605No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5711.6502413225051298No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4611.3323314355076443No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4461.2889800872807144No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4251.2282881997630126No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4081.1791566717724922No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3410.9855206496922054No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3380.9768503800468195No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3280.9479494812288661No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2910.8410161556024393No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2780.8034449871391001No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2690.7774341782029421No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2620.7572035490303749No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2500.7225224704488309No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2420.6994017513944684No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2380.687841391867287No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2380.687841391867287No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2320.6705008525765151No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2310.6676107626947198No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2270.6560504031675385No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2140.6184792347041993No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1990.5751278864772694No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1900.5491170775411116No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1840.5317765382503395No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1770.5115459090777723No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1740.5028756394323863No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1700.49131527990520507No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1700.49131527990520507No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1680.4855351001416144No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1660.47975492037802375No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1640.47397474061443307No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1630.47108465073263783No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1620.4681945608508425No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1580.45663420132366117No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1570.4537441114418658No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1550.44796393167827514No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1540.44507384179647985No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1450.4190630328603219No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1430.41328285309673135No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1430.41328285309673135No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1420.410392763214936No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC1380.3988324036877547No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1350.3901621340423687No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1340.38727204416057337No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1310.37860177451518745No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1300.3757116846333921No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1280.3699315048698015No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1250.36126123522441544No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1190.3439206959336435No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1160.3352504262882576No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1160.3352504262882576No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1130.3265801566428716No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1070.30923961735209965No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1050.30345943758850896No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1050.30345943758850896No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1030.29767925782491833No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1020.29478916794312304No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1010.2918990780613277No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1010.2918990780613277No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1000.2890089881795324No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT970.2803387185341464No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT960.2774486286523511No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT950.2745585387705558No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT950.2745585387705558No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT890.2572179994797838No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT870.25143781971619317No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT860.24854772983439788No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC840.2427675500708072No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT840.2427675500708072No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC830.23987746018901188No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT780.22542701078003524No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT740.21386665125285395No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT740.21386665125285395No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC720.20808647148926335No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG710.205196381607468No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT700.20230629172567266No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC700.20230629172567266No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT690.19941620184387734No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG690.19941620184387734No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT680.196526111962082No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC680.196526111962082No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT650.18785584231669605No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC640.18496575243490074No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT630.1820756625531054No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT620.17918557267131008No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT620.17918557267131008No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT620.17918557267131008No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT620.17918557267131008No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC600.17340539290771942No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA600.17340539290771942No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC600.17340539290771942No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT590.1705153030259241No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT590.1705153030259241No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT580.1676252131441288No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC580.1676252131441288No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT580.1676252131441288No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC560.16184503338053813No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC550.1589549434987428No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA540.1560648536169475No Hit
CCTACGGGTGGCAGGCGGGCGGACGCTCCCTACAAAGCCTTCAGGAGCAT520.15028467385335684No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT510.14739458397156152No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC510.14739458397156152No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT500.1445044940897662No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT490.14161440420797086No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC490.14161440420797086No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC490.14161440420797086No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC480.13872431432617555No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC470.13583422444438023No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCGCAATGGACGAAAGTCTGAC470.13583422444438023No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC470.13583422444438023No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC470.13583422444438023No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT470.13583422444438023No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT460.1329441345625849No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA460.1329441345625849No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG450.13005404468078957No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC450.13005404468078957No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG450.13005404468078957No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT440.12716395479899426No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT440.12716395479899426No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT420.1213837750354036No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT420.1213837750354036No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT420.1213837750354036No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA410.11849368515360827No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA410.11849368515360827No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTGCGCAATGGGCGAAAGCCTGAC400.11560359527181295No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT400.11560359527181295No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC390.11271350539001762No Hit
CCTACGGGTGGCAGCGAAGGCCATCCCACCGGTGGCCCAGGCGGCTGCTA390.11271350539001762No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC380.1098234155082223No Hit
CCTACGGGAGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC380.1098234155082223No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA380.1098234155082223No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCCACTCC380.1098234155082223No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT380.1098234155082223No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC380.1098234155082223No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT380.1098234155082223No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC370.10693332562642698No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT370.10693332562642698No Hit
CCTACGGGTGGCAGCAGGGGTTGGGAGGAGCAGCAGGGGTTGGGGGGAGC360.10404323574463167No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT360.10404323574463167No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT360.10404323574463167No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT350.10115314586283633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)