FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27451
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG13484.910567921022914No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9493.4570689592364574No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG7982.9069979235729115No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG6252.2767840880113654No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG6202.2585698153072746No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC5912.1529270336235475No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5862.1347127609194563No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5552.0217842701540927No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4451.6210702706640923No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3971.4462132527048195No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3631.3223561983170011No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3541.2895705074496375No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3521.2822847983680012No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3341.2167134166332738No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2781.0127135623474555No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2440.8888565079596372No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2150.7832137262759099No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2040.7431423263269098No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1990.7249280536228188No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1830.6666423809697279No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1790.6520709628064552No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1520.5537138902043641No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1480.5391424720410913No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1260.4589996721430913No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1020.3715711631634549No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG970.35335689045936397No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT900.32785690867363665No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT860.3132854905103639No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC750.27321409056136386No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA600.2185712724490911No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC580.21128556336745477No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC580.21128556336745477No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC570.20764270882663657No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC560.20399985428581838No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG540.196714145204182No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC540.196714145204182No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC530.19307129066336381No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCGAGATTGTGGCACTGTACTCC520.18942843612254562No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT510.18578558158172745No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA510.18578558158172745No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT510.18578558158172745No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC500.18214272704090925No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT490.17849987250009108No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC490.17849987250009108No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT480.1748570179592729No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC480.1748570179592729No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT470.1712141634184547No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA470.1712141634184547No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC470.1712141634184547No Hit
CCTACGGGTGGCAGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT450.16392845433681832No Hit
CCTACGGGTGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG450.16392845433681832No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG440.16028559979600016No Hit
CCTACGGGTGGCAGGCGGGCGGACGCTCCCTACAAAGCCTTCAGGAGCAT420.1529998907143638No Hit
CCTACGGGTGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC420.1529998907143638No Hit
CCTACGGGTGGCAGCGAAGGCCATCCCACCGGTGGCCCAGGCGGCTGCTA390.14207132709190923No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT390.14207132709190923No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC390.14207132709190923No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT390.14207132709190923No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT380.13842847255109103No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT380.13842847255109103No Hit
CCTACGGGAGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC380.13842847255109103No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT370.13478561801027283No Hit
CCTACGGGTGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA370.13478561801027283No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT370.13478561801027283No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC360.13114276346945467No Hit
CCTACGGGTGGCTGCAGTCACAGCTTCAGTGGACTGAATCTTTCCTGCCT360.13114276346945467No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT360.13114276346945467No Hit
CCTACGGGGGGCAGAGCTTGCAGTGAGCCGAGATTGTGGCACTGTACTCC350.12749990892863647No Hit
CCTACGGGGGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC350.12749990892863647No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC350.12749990892863647No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC340.1238570543878183No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC340.1238570543878183No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT340.1238570543878183No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC340.1238570543878183No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT330.1202141998470001No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT330.1202141998470001No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC320.11657134530618192No Hit
CCTACGGGAGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC320.11657134530618192No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC320.11657134530618192No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC320.11657134530618192No Hit
CCTACGGGAGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT320.11657134530618192No Hit
CCTACGGGTGGCTGAGGCAGGAGAAGCACTTGAACCTGGGAGGCGGAGGT320.11657134530618192No Hit
CCTACGGGAGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG310.11292849076536372No Hit
CCTACGGGCGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG310.11292849076536372No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCCACTCC310.11292849076536372No Hit
CCTACGGGAGGCAGAGCTTGCAGTGAGCCGAGATTGTGGCACTGTACTCC310.11292849076536372No Hit
CCTACGGGTGGCTGCCCTGAGTCATAGAACCACAAGGAATCTAGTTTCCT310.11292849076536372No Hit
CCTACGGGCGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA300.10928563622454555No Hit
CCTACGGGCGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG300.10928563622454555No Hit
CCTACGGGAGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC300.10928563622454555No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT300.10928563622454555No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC300.10928563622454555No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTAAGTCGGGAGGGGAAATG290.10564278168372739No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC290.10564278168372739No Hit
CCTACGGGCGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA290.10564278168372739No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT290.10564278168372739No Hit
CCTACGGGTGGCAGCAGGGGTTGGGAGGAGCAGCAGGGGTTGGGGGGAGC280.10199992714290919No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT280.10199992714290919No Hit
CCTACGGGAGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA280.10199992714290919No Hit
CCTACGGGTGGCAGGAGCTTAGTGTGCCTAGAGGAAGATGTGTCTACAAA280.10199992714290919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)