FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32019
Sequences flagged as poor quality0
Sequence length85-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG9592.9950966613573193No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8732.7265061369811674No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG6812.1268621755832475No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6712.095630719260439No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5691.777069864767794No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG5071.5834348355663823No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4591.4335238452169023No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG4551.4210312626877792No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC4091.2773665636028608No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3901.2180267965895248No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3751.1711796121053124No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3591.1212092819888193No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3401.0618695149754833No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3241.01189918485899No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3050.9525594178456541No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2840.8869733595677566No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2780.8682344857740717No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2780.8682344857740717No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2710.8463724663481059No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2630.8213873012898592No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2560.7995252818638934No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2530.7901558449670508No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2320.7245697866891533No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC2250.7027077672631875No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2240.6995846216309066No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2140.6683531653080983No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2110.6589837284112559No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1910.5965208157656392No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1880.5871513788687966No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1880.5871513788687966No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1820.5684125050751117No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1800.56216621381055No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1770.5527967769137075No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1670.5215653205908992No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1660.5184421749586183No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1600.49970330116493333No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1570.4903338642680909No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1570.4903338642680909No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1560.48721071863580995No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1560.48721071863580995No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1540.48096442737124834No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1510.4715949904744058No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1490.4653486992098442No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1450.45285611668072084No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1440.44973297104844No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1400.4372403885193167No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1370.4278709516224741No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1350.4216246603579125No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1310.40913207782878913No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1300.40600893219650835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1230.38414691277054247No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1220.3810237671382617No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1220.3810237671382617No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1190.37165433024141914No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC1160.36228489334457664No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1150.35916174771229586No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1150.35916174771229586No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC1130.35291545644773414No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1100.3435460195508917No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1070.33417658265404915No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1010.31543770886036415No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC950.29669883506667916No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT940.2935756894343983No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT940.2935756894343983No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG920.28732939816983666No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT900.281083106905275No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT900.281083106905275No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT880.27483681564071333No Hit
CCTACGGGTGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG860.26859052437615166No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT860.26859052437615166No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC840.26234423311159No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT830.25922108747930916No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC830.25922108747930916No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC830.25922108747930916No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC810.2529747962147475No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT810.2529747962147475No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA800.24985165058246667No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG790.24672850495018583No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT770.24048221368562417No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT770.24048221368562417No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT760.2373590680533433No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC750.2342359224210625No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG750.2342359224210625No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT740.23111277678878167No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT740.23111277678878167No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT740.23111277678878167No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT720.22486648552422No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC680.21237390299509665No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT680.21237390299509665No Hit
CCTACGGGGGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC660.20612761173053498No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT640.19988132046597334No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT640.19988132046597334No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT640.19988132046597334No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT640.19988132046597334No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT620.19363502920141165No Hit
CCTACGGGCGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG620.19363502920141165No Hit
CCTACGGGAGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG610.19051188356913085No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT600.18738873793685001No Hit
CCTACGGGAGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG590.18426559230456915No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT580.18114244667228832No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT570.1780193010400075No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT560.17489615540772666No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC550.17177300977544585No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT550.17177300977544585No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC550.17177300977544585No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT540.168649864143165No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT540.168649864143165No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT540.168649864143165No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC520.16240357287860333No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT510.1592804272463225No Hit
CCTACGGGCGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC510.1592804272463225No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG500.15615728161404166No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC500.15615728161404166No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT490.15303413598176083No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC490.15303413598176083No Hit
CCTACGGGGGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC480.14991099034948No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC480.14991099034948No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT470.14678784471719916No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT470.14678784471719916No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC460.14366469908491833No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT460.14366469908491833No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC460.14366469908491833No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC460.14366469908491833No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG450.1405415534526375No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT440.13741840782035666No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC430.13429526218807583No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC430.13429526218807583No Hit
CCTACGGGGGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG430.13429526218807583No Hit
CCTACGGGCGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC420.131172116555795No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT420.131172116555795No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC410.12804897092351417No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT410.12804897092351417No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG410.12804897092351417No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT410.12804897092351417No Hit
CCTACGGGTGGCAGCAGGGGTTGGGAGGAGCAGCAGGGGTTGGGGGGAGC410.12804897092351417No Hit
CCTACGGGTGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC410.12804897092351417No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT410.12804897092351417No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT400.12492582529123333No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA400.12492582529123333No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC400.12492582529123333No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT390.12180267965895249No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT390.12180267965895249No Hit
CCTACGGGCGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA390.12180267965895249No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT380.11867953402667165No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT380.11867953402667165No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT370.11555638839439084No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC370.11555638839439084No Hit
CCTACGGGAGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC370.11555638839439084No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT360.11243324276211No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC360.11243324276211No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT350.10931009712982917No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGGAACCCTGAT350.10931009712982917No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC340.10618695149754832No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT340.10618695149754832No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT340.10618695149754832No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT340.10618695149754832No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG340.10618695149754832No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT340.10618695149754832No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC330.10306380586526749No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT330.10306380586526749No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTTCGCAATGGGCGCAAGCCTGAC330.10306380586526749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)