FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49151
Sequences flagged as poor quality0
Sequence length39-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG19443.9551585929075705No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13632.773087017558137No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG13322.71001607291815No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12432.5289414254033487No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10312.0976175459298894No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG10272.08947935952473No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10132.060995707106671No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG10052.044719334296352No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10042.042684787695062No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8021.631706374234502No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC7341.4933572053467885No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7171.4587699131248602No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6691.361111676262945No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6621.3468698500539156No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5911.2024170413623323No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5551.1291733637158958No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5211.0599987792720393No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4600.9358914365933552No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4430.9013041443714268No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4340.8829932249598178No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4180.8504404793391793No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4100.8341641065288601No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3060.6225712599947102No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC3010.6123985269882607No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2380.4842220911069968No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT2210.44963479888506847No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2060.41911659986572No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1920.39063294744766125No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1920.39063294744766125No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1900.3865638542450815No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1830.3723220280360522No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1780.36214929502960264No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1760.3580802018270228No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1480.3011128969909056No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC1440.292974710585746No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1340.27262924457284693No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG1330.27059469797155705No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1320.2685601513702671No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1300.2644910581676873No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1300.2644910581676873No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1290.26245651156639743No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1280.2604219649651075No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1260.2563528717625277No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1210.24618013875607822No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1190.2421110455534984No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1130.229903765945759No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1110.2258346727431792No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1100.2238001261418893No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC1080.21973103293930948No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1050.2136273931354398No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1040.2115928465341499No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1010.20548920673028015No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT1010.20548920673028015No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1000.20345466012899024No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT970.19735102032512053No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG970.19735102032512053No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC970.19735102032512053No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC920.18717828731867103No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC900.18310919411609122No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC890.18107464751480132No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT890.18107464751480132No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT880.1790401009135114No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC880.1790401009135114No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT880.1790401009135114No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC870.1770055543122215No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT870.1770055543122215No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT840.17090191450835182No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC840.17090191450835182No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT830.16886736790706192No Hit
CCTACGGGAGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG830.16886736790706192No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA820.166832821305772No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT820.166832821305772No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC810.1647982747044821No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT780.1586946349006124No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT770.1566600882993225No Hit
CCTACGGGTGGCAGAGCTTGCAGTGAGCCAAGATTGTGGCACTGTACTCC770.1566600882993225No Hit
CCTACGGGCGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG760.1546255416980326No Hit
CCTACGGGCGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG750.1525909950967427No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT730.1485219018941629No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG730.1485219018941629No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT730.1485219018941629No Hit
CCTACGGGTGGCAGAGGCTGCGGTGAGCCGAGATGGCGCCACTGCACTTC700.14241826209029318No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG690.14038371548900327No Hit
CCTACGGGTGGCAGAGGTTGCAGTAAGCCAAGATTGCACCATTGCACTCC690.14038371548900327No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC680.13834916888771337No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT670.13631462228642346No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT660.13428007568513356No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT660.13428007568513356No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC660.13428007568513356No Hit
CCTACGGGGGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC650.13224552908384365No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC630.12817643588126384No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG630.12817643588126384No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC630.12817643588126384No Hit
CCTACGGGCGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC610.12410734267868405No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC610.12410734267868405No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC600.12207279607739416No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT590.12003824947610425No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT580.11800370287481435No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT580.11800370287481435No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT580.11800370287481435No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT570.11596915627352444No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC560.11393460967223455No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA560.11393460967223455No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC560.11393460967223455No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC560.11393460967223455No Hit
CCTACGGGGGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG550.11190006307094465No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC540.10986551646965474No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT540.10986551646965474No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC530.10783096986836484No Hit
CCTACGGGTGGCTGAGGCAGGAGAATTGCGTGAACCCGGGAGGCAGAGGT530.10783096986836484No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT530.10783096986836484No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT510.10376187666578504No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA510.10376187666578504No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT510.10376187666578504No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC500.10172733006449512No Hit
CCTACGGGTGGCAGGCGGGCGGACGCTCCCTACAAAGCCTTCAGGAGCAT500.10172733006449512No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC500.10172733006449512No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT500.10172733006449512No Hit
CCTACGGGTGGCAGCGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC500.10172733006449512No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT500.10172733006449512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)