FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31645
Sequences flagged as poor quality0
Sequence length42-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG14944.721124980249645No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG9352.9546531837573076No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8042.540685732343182No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG7722.4395639121504185No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG7282.300521409385369No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7092.2404803286459156No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6892.1772791910254385No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6692.114078053404961No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6161.9465950387106967No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5391.7032706588718596No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4841.5294675304155474No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC4371.380944857007426No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4311.3619845157212829No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4011.2671828092905673No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3911.2355822404803287No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3781.1945015010270186No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3761.1881813872649707No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3251.027018486332754No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3241.0238584294517301No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3130.9890978037604676No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3100.9796176331173961No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3010.9511771211881814No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2850.9006162110917997No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2600.8216147890662032No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2510.7931742771369885No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2350.7426133670406068No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2070.6541317743719387No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2020.6383314899668194No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1670.5277294991309843No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1560.4929688734397219No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1560.4929688734397219No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1470.4645283615105072No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1370.4329277927002686No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1270.40132722389003006No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1160.3665665981987676No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1100.3476062569126244No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1100.3476062569126244No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG1090.34444620003160054No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC1050.3318059725075051No Hit
CCTACGGGTGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1030.32548585874545743No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1010.3191657449834097No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1000.31600568810238583No Hit
CCTACGGGTGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC950.30020540369726656No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG910.2875651761731711No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT870.2749249486490757No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC820.2591246642439564No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG790.2496444936008848No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT760.24016432295781323No Hit
CCTACGGGAGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG730.23068415231474165No Hit
CCTACGGGAGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC730.23068415231474165No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC720.22752409543371782No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG700.2212039816716701No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT690.21804392479064624No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC690.21804392479064624No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG690.21804392479064624No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC620.19592352662347923No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG600.1896034128614315No Hit
CCTACGGGAGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC570.18012324221835993No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT560.17696318533733607No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC540.17064307157528835No Hit
CCTACGGGGGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG540.17064307157528835No Hit
CCTACGGGTGGCTGCATCATGGGGAGCCATTTTACAAGCAGCACCATGGG530.1674830146942645No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC530.1674830146942645No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC500.15800284405119291No Hit
CCTACGGGTGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC500.15800284405119291No Hit
CCTACGGGCGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC490.15484278717016906No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT490.15484278717016906No Hit
CCTACGGGGGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC480.1516827302891452No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT480.1516827302891452No Hit
CCTACGGGGGGCAGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC470.14852267340812134No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT470.14852267340812134No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG460.14536261652709748No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC440.13904250276504979No Hit
CCTACGGGAGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG430.13588244588402593No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC430.13588244588402593No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT430.13588244588402593No Hit
CCTACGGGTGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC410.1295623321219782No Hit
CCTACGGGCGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCAGTTAC400.12640227524095435No Hit
CCTACGGGGGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG400.12640227524095435No Hit
CCTACGGGCGGCAGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG390.12324221835993047No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT380.12008216147890662No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC380.12008216147890662No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC380.12008216147890662No Hit
CCTACGGGAGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA380.12008216147890662No Hit
CCTACGGGAGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC380.12008216147890662No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC370.11692210459788276No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC370.11692210459788276No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT370.11692210459788276No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT370.11692210459788276No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT360.11376204771685891No Hit
CCTACGGGTGGCTGCAGTGGGAGGACCACTTGAGCCTAGGAATTCAAGGC360.11376204771685891No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT350.11060199083583505No Hit
CCTACGGGGGGCTGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC350.11060199083583505No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT350.11060199083583505No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC340.10744193395481119No Hit
CCTACGGGAGGCAGCAACCATTGCAGAGTGGCAGCCAGCCTAGGCAAGCC340.10744193395481119No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGTGGAGGT340.10744193395481119No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT340.10744193395481119No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC340.10744193395481119No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA330.10428187707378733No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT320.10112182019276347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)