FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31645
Sequences flagged as poor quality0
Sequence length42-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC7632.4111234002212036No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6362.0097961763311742No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC6061.9149944699004582No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5811.8359930478748616No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5641.7822720808974561No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5521.74435139832517No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5511.7411913414441462No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4801.516827302891452No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4651.4694264496760943No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4521.4283457102227841No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4451.406225312055617No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4331.3683046294833308No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3861.2197819560752092No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3311.0459788276188973No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3140.9922578606414916No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3070.9701374624743245No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2940.9290567230210144No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2750.869015642281561No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2680.8468952441143941No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2630.8310949597092748No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2570.8121346184231316No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2570.8121346184231316No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2570.8121346184231316No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2540.8026544477800601No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2460.7773739927318691No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2380.7520935376836784No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2380.7520935376836784No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2370.7489334808026544No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2280.7204929688734397No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2230.7046926844683205No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2140.6762521725391056No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2110.6667720018960341No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2090.6604518881339864No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2080.6572918312529626No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2000.6320113762047717No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1990.6288513193237478No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1900.6004108073945331No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1860.5877705798704377No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1740.5498498972981514No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1710.5403697266550798No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1700.5372096697740559No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1600.5056091009638174No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1590.5024490440827936No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1480.467688418391531No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1430.45188813398641176No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1360.42976773581924477No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1350.4266076789382209No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1300.4108073945331016No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1300.4108073945331016No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1210.38236688260388685No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1170.36972665507979147No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1170.36972665507979147No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1060.334966029388529No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1030.32548585874545743No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1020.3223258018644336No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA960.3033654605782904No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA920.29072523305419495No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG910.2875651761731711No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG890.2812450624111234No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA860.27176489176805185No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG810.25596460736293253No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA810.25596460736293253No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG800.2528045504819087No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT790.2496444936008848No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT790.2496444936008848No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG780.24648443671986095No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG750.23700426607678937No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA750.23700426607678937No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG740.2338442091957655No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT670.21172381102859852No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG670.21172381102859852No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT660.20856375414757466No Hit
GACTACCAGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC660.20856375414757466No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG620.19592352662347923No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG590.18644335598040765No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT570.18012324221835993No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG550.1738031284563122No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG540.17064307157528835No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG540.17064307157528835No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT540.17064307157528835No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG540.17064307157528835No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG530.1674830146942645No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT530.1674830146942645No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT530.1674830146942645No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG510.1611629009322168No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG510.1611629009322168No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG480.1516827302891452No Hit
GACTACACGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG480.1516827302891452No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG470.14852267340812134No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG450.14220255964607362No Hit
GACTACTAGGGTATCTAATCCTCCGAAGGGTGGCTGGGGAGTTCTGTTGC440.13904250276504979No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA430.13588244588402593No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG420.13272238900300207No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG420.13272238900300207No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG420.13272238900300207No Hit
GACTACCAGGGTATCTAATCCCAGTTTGCATCTTCGCTATTGTGTATTCA410.1295623321219782No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG410.1295623321219782No Hit
GACTACAGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG400.12640227524095435No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG400.12640227524095435No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG400.12640227524095435No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT400.12640227524095435No Hit
GACTACCGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG400.12640227524095435No Hit
GACTACCGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT400.12640227524095435No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG380.12008216147890662No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA370.11692210459788276No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG370.11692210459788276No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT370.11692210459788276No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG340.10744193395481119No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG340.10744193395481119No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG330.10428187707378733No Hit
GACTACACGGGTATCTAATCCTGGACGGCTGTCTGACATCACCCCGTTTC330.10428187707378733No Hit
GACTACTAGGGTATCTAATCCACACAGCAGAAATCAGAAACATCTGGTAT330.10428187707378733No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA320.10112182019276347No Hit
GACTACTGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT320.10112182019276347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)