FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22451
Sequences flagged as poor quality0
Sequence length46-251
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4732.106810387065164No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3721.6569417843303194No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3361.4965925793951274No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3311.4743218564874616No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3271.4565052781613292No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3231.4386886998351966No Hit
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3121.3896931094383325No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2861.2738853503184715No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2621.1669858803616766No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2551.1358068682909446No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2541.1313527237094116No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2411.0734488441494812No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2381.0600864104048817No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2351.0467239766602825No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2281.0155449645895507No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2220.9888200971003519No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2170.9665493741926863No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2020.8997372054696896No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2000.8908289163066234No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1880.837379181328226No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1810.806200169257494No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1770.7883835909313617No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1720.766112868023696No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1630.726025566789898No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1540.6859382655560999No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1530.6814841209745668No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1520.6770299763930337No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1390.6191260968331033No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1390.6191260968331033No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1350.6013095185069708No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1280.5701305064362389No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1270.5656763618547058No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG1260.5612222172731727No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1190.5300432052024409No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1180.5255890606209078No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1180.5255890606209078No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1170.5211349160393747No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1130.5033183377132422No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1120.49886419313170904No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1100.4899559039686428No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1070.47659347022404347No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1050.46768518106097723No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1000.4454144581533117No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG940.418689590664113No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA930.41423544608257984No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA900.4008730123379805No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC890.39641886775644736No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT890.39641886775644736No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG880.39196472317491426No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA870.3875105785933811No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG860.383056434011848No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG840.37414814484878184No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT840.37414814484878184No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC810.36078571110418245No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA810.36078571110418245No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA790.3518774219411162No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA780.3474232773595831No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA780.3474232773595831No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG750.3340608436149837No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA740.32960669903345063No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG740.32960669903345063No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG740.32960669903345063No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG730.3251525544519175No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG720.3206984098703844No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG710.3162442652888513No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG710.3162442652888513No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT690.30733597612578506No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA690.30733597612578506No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG680.3028818315442519No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG680.3028818315442519No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG670.2984276869627188No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA660.29397354238118567No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA640.28506525321811943No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT640.28506525321811943No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG630.28061110863658634No Hit
GACTACACGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG620.2761569640550532No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT610.27170281947352015No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG600.267248674891987No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT590.2627945303104539No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG580.25834038572892076No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT580.25834038572892076No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA570.25388624114738767No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG560.24943209656585452No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA540.24052380740278828No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG540.24052380740278828No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG530.23606966282125516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG520.23161551823972204No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA520.23161551823972204No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG510.22716137365818892No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT510.22716137365818892No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA470.2093447953320565No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA460.20489065075052337No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT450.20043650616899025No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG440.19598236158745713No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA430.191528217005924No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG430.191528217005924No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA430.191528217005924No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA430.191528217005924No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG420.18707407242439092No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA390.17371163867979156No Hit
GACTACTGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG390.17371163867979156No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG390.17371163867979156No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA380.16925749409825844No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG380.16925749409825844No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG370.16480334951672532No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG370.16480334951672532No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG370.16480334951672532No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA360.1603492049351922No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG360.1603492049351922No Hit
GACTACAGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG360.1603492049351922No Hit
GACTACTCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT360.1603492049351922No Hit
GACTACCAGGGTATCTAATCACTAGAGCTTGCTTTCCTGTAAAGATTGTC350.15589506035365908No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG350.15589506035365908No Hit
GACTACCGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG340.15144091577212596No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG340.15144091577212596No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA340.15144091577212596No Hit
GACTACTGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT330.14698677119059284No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG330.14698677119059284No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT330.14698677119059284No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA330.14698677119059284No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA320.14253262660905971No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG320.14253262660905971No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA320.14253262660905971No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG310.1380784820275266No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA310.1380784820275266No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG310.1380784820275266No Hit
GACTACCCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT300.1336243374459935No Hit
GACTACAGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT300.1336243374459935No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA300.1336243374459935No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA290.12917019286446038No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG290.12917019286446038No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG290.12917019286446038No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG270.12026190370139414No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA270.12026190370139414No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG270.12026190370139414No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG260.11580775911986102No Hit
GACTACAAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT260.11580775911986102No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG260.11580775911986102No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA250.11135361453832793No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA250.11135361453832793No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA250.11135361453832793No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG250.11135361453832793No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG240.10689946995679481No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAT240.10689946995679481No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG240.10689946995679481No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA240.10689946995679481No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG240.10689946995679481No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG240.10689946995679481No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG230.10244532537526169No Hit
GACTACCAGGGTATCTTGAGGGTGGTGCCCAGGCCTGTCCTACTGGCCCT230.10244532537526169No Hit
GACTACTCGGGTATCTTGAGGGTGGTGCCCAGGCCTGTCCTACTGGCCCT230.10244532537526169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)