FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48319
Sequences flagged as poor quality0
Sequence length50-251
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5521.142407748504729No Hit
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC5491.1361990107411164No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4810.9954676214325627No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4780.9892588836689501No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4730.9789109873962624No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4650.9623543533599619No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4360.902336554978373No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4290.8878495001966101No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4110.8505970736149341No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4020.8319708603240961No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3940.8154142262877957No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3910.8092054885241831No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3820.790579275233345No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3730.7719530619425071No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3720.7698834826879696No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3690.7636747449243568No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3660.7574660071607443No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3610.7471181108880565No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3560.7367702146153686No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3510.726422318342681No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3500.7243527390881433No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3440.7119352635609181No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3420.707796105051843No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3390.7015873672882303No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3370.6974482087791553No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3320.6871003125064674No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3210.6643349407065543No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3180.6581262029429418No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3050.6312216726339536No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3030.6270825141248784No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3010.6229433556158033No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2980.6167346178521906No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2980.6167346178521906No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2980.6167346178521906No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2950.610525880088578No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2810.5815517705250522No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2790.5774126120159772No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2740.5670647157432894No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2740.5670647157432894No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2570.531881868416151No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2570.531881868416151No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.5194643928889257No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2490.5153252343798506No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2480.513255655125313No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2470.5111860758707755No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2460.5091164966162379No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2280.471864070034562No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2270.46979449078002444No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2240.46358575301641175No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2220.45944659450733666No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2220.45944659450733666No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2210.45737701525279906No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2160.44702911898011133No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2150.4449595397255738No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2080.43047248494381096No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2050.42426374718019827No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2010.41598543016204803No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1840.3808025828349097No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1820.37666342432583455No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1810.374593845071297No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1810.374593845071297No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1810.374593845071297No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1790.37045468656222186No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1780.3683851073076843No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1740.36010679028953413No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1710.3538980525259215No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1690.3497588940168464No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1660.3435501562532337No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1660.3435501562532337No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1640.3394109977441586No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1630.33734141848962107No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1630.33734141848962107No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1590.3290631014714709No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1550.32078478445332065No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1530.3166456259442455No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1470.3042281504170202No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1460.3021585711624827No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1460.3021585711624827No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1450.30008899190794514No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1450.30008899190794514No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1420.29388025414433244No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1420.29388025414433244No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1410.2918106748897949No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1400.28974109563525735No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1370.2835323578716447No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1360.28146277861710717No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1310.2711148823444194No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1260.2607669860717316No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1240.2566278275626565No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1210.2504190897990438No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1200.2483495105445063No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1180.24421035203543118No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1150.23800161427181854No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1150.23800161427181854No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1100.22765371799913078No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1080.22351455949005566No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1050.21730582172644303No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT980.20281876694468012No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG980.20281876694468012No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG980.20281876694468012No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG970.20074918769014258No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG960.19867960843560503No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG930.1924708706719924No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT930.1924708706719924No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT880.1821229743993046No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT880.1821229743993046No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG850.17591423663569197No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT850.17591423663569197No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT840.1738446573811544No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT830.17177507812661685No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG820.1697054988720793No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG800.16556634036300422No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG790.16349676110846667No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.15935760259939152No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG750.15521844409031643No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT720.14900970632670377No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG700.14487054781762868No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG690.14280096856309113No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT650.13452265154494092No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG640.13245307229040337No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG630.1303834930358658No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT620.12831391378132825No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTCAG620.12831391378132825No Hit
GACTACCAGGGTATCTAATCAAACACCTACCATTGGTCTGGCACTCTCCT600.12417475527225315No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG600.12417475527225315No Hit
GACTACAAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT560.11589643825410294No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG560.11589643825410294No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG560.11589643825410294No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.11382685899956539No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG530.10968770049049029No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG530.10968770049049029No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG510.10554854198141518No Hit
GACTACTAGGGTATCTAATCACTAGAGCTTGCTTTCCTGTAAAGATTGTC510.10554854198141518No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA500.10347896272687764No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG500.10347896272687764No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG490.10140938347234006No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG490.10140938347234006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)