FastQCFastQC Report
Tue 7 May 2024
EGAF00004835770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21564
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length35-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5522.559821925431274No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5062.346503431645335No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4992.3140419217213872No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3601.669449081803005No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3581.6601743646818772No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3431.5906139862734185No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3281.5210536078649601No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2991.386570209608607No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2981.381932851048043No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2891.340196624002968No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2881.335559265442404No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2821.3077351140790205No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2731.2659988870339456No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2691.24744945279169No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2631.2196253014283065No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2541.1778890743832313No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2481.1500649230198479No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2371.099053978853645No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2341.0851419031719534No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2271.052680393248006No Hit
CCTACGGGTGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC2150.9970320905212391No Hit
CCTACGGGTGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG2140.9923947319606752No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2050.9506585049156No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2050.9506585049156No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2010.9321090706733445No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1800.8347245409015025No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1750.8115377480986831No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1650.765164162493044No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1650.765164162493044No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1630.7558894453719162No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1620.7512520868113522No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1550.7187905768874049No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1450.6724169912817659No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1440.667779632721202No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1410.6538675570395103No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1390.6445928399183825No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1380.6399554813578185No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1330.616768688554999No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1320.6121313299944352No Hit
CCTACGGGAGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1290.5982192543127435No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1280.5935818957521796No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1270.5889445371916157No Hit
CCTACGGGAGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1250.5796698200704878No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1250.5796698200704878No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1210.5611203858282322No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1200.5564830272676683No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1190.5518456687071045No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1180.5472083101465406No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1160.5379335930254127No Hit
CCTACGGGGGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1110.5147468002225932No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT1100.5101094416620293No Hit
CCTACGGGCGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC1090.5054720831014654No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.5008347245409015No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1030.47764793173808195No Hit
CCTACGGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGAGGGGAAATG1020.47301057317751805No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC960.4451864218141347No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT940.43591170469300683No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT920.42663698757187907No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT900.41736227045075125No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC830.38490076052680394No Hit
CCTACGGGGGGCAGAGGTTGCAGTGAGTTGAGATCCTGCCATTCAACTCC810.3756260434056761No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC750.34780189204229267No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC750.34780189204229267No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC590.2736041550732703No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC560.25969207939157857No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC550.25505472083101466No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC540.25041736227045075No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT540.25041736227045075No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC540.25041736227045075No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC530.24578000370988687No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT520.24114264514932293No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC480.22259321090706735No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC460.21331849378593953No Hit
CCTACGGGGGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT450.20868113522537562No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT450.20868113522537562No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC430.1994064181042478No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC400.1854943424225561No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA400.1854943424225561No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT400.1854943424225561No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT390.1808569838619922No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG380.17621962530142832No Hit
CCTACGGGCGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGCT360.1669449081803005No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC350.1623075496197366No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC340.1576701910591727No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT320.1483954739380449No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC320.1483954739380449No Hit
CCTACGGGTGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT320.1483954739380449No Hit
CCTACGGGTGGCTGCAGGGAGGAAAACCTGCAGTCACTCACCCCATTTAC300.13912075681691707No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT300.13912075681691707No Hit
CCTACGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCGGAGGT290.13448339825635316No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT280.12984603969578928No Hit
CCTACGGGTGGCTGCTGGGGCCTGACTCCAGCTGGGAGGGTTATGAACTG270.12520868113522537No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT270.12520868113522537No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGAT270.12520868113522537No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC270.12520868113522537No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC260.12057132257466147No Hit
CCTACGGGTGGCGCTGTCAGGCTCCTTGCGGTGCTGCTGGTGGAGCGCCA260.12057132257466147No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG250.11593396401409757No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT250.11593396401409757No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT240.11129660545353368No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT240.11129660545353368No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA240.11129660545353368No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT240.11129660545353368No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA230.10665924689296977No Hit
CCTACGGGTGGCAGACAAGGCCTGATCGCCACCACTCCTCCCTCCTCAGA230.10665924689296977No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG230.10665924689296977No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGACGGAAGTCTGAC230.10665924689296977No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGAGGCAACTCTGAA230.10665924689296977No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC230.10665924689296977No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT230.10665924689296977No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT220.10202188833240587No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT220.10202188833240587No Hit
CCTACGGGTGGCCCAGGCGGCTGCTACACAATGTTTCCCTTTAGTCGGGA220.10202188833240587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)