FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28949
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC4921.699540571349615No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4821.6649970638018585No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3781.3057445853051919No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3731.2884728315313136No Hit
GACTACTAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3711.2815641300217624No Hit
GACTACAAGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3541.2228401671905764No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3481.2021140626619227No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3231.1157552937925317No Hit
GACTACCCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC3051.0535769802065702No Hit
GACTACTCGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2911.0052160696397112No Hit
GACTACCAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2890.9983073681301599No Hit
GACTACACGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC2750.9499464575633009No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2630.9084942485059934No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2570.8877681439773395No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2550.8808594424677882No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2470.853224636429583No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2430.8394072334104805No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2310.7979550243531728No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2200.7599571660506408No Hit
GACTACTAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2190.7565028152958652No Hit
GACTACCCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2150.7426854122767625No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2100.7254136585028843No Hit
GACTACAAGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT2100.7254136585028843No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1980.6839614494455767No Hit
GACTACCGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1930.6666896956716986No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1800.6217831358596152No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1740.6010570313309613No Hit
GACTACTCGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1710.5906939790666343No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1700.5872396283118588No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1700.5872396283118588No Hit
GACTACACGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT1610.5561504715188781No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1540.5319700162354486No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1500.518152613216346No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1490.5146982624615704No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1470.5077895609520191No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1430.4939721579329165No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1430.4939721579329165No Hit
GACTACTGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1400.4836091056685896No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1380.4767004041590383No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA1330.4594286503851601No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1320.45597429963038444No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1310.4525199488756088No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.4456112473660575No Hit
GACTACAGGGGTATCTAATCCTGGAAGGCTGTCTGACATCACCCCGTTTC1180.40761338906352546No Hit
GACTACCAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1140.39379598604442295No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1130.3903416352896473No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1130.3903416352896473No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1110.383432933780096No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1080.3730698815157691No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG970.3350720232132371No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG960.3316176724584614No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG960.3316176724584614No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG940.32470897094891016No Hit
GACTACAAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT910.3143459186845832No Hit
GACTACCGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT910.3143459186845832No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG860.29707416491070504No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG840.29016546340115373No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG810.27980241113682686No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA790.27289370962727555No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG790.27289370962727555No Hit
GACTACAGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT770.2659850081177243No Hit
GACTACCAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG770.2659850081177243No Hit
GACTACTAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG750.259076306608173No Hit
GACTACACGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT750.259076306608173No Hit
GACTACAAGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG750.259076306608173No Hit
GACTACCCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT750.259076306608173No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG740.25562195585339736No Hit
GACTACTGGGGTATCTAATCCAGTCCAGCATCGTGCTTATAGAGAGATTT730.2521676050986217No Hit
GACTACTAGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT720.24871325434384609No Hit
GACTACCCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG700.2418045528342948No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG680.2348958513247435No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT620.21416974679608966No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG610.210715396041314No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG590.20380669453176273No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG580.20035234377698713No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG580.20035234377698713No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA580.20035234377698713No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG550.1899892915126602No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG530.18308059000310892No Hit
GACTACCGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT530.18308059000310892No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG510.17617188849355764No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG500.172717537738782No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG500.172717537738782No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT480.1658088362292307No Hit
GACTACTCGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG450.1554457839649038No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA450.1554457839649038No Hit
GACTACACGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG450.1554457839649038No Hit
GACTACCAGGGTATCTTGAGGGTGGTGCCCAGGCCTGTCCTACTGGCCCT430.14853708245535252No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG430.14853708245535252No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG430.14853708245535252No Hit
GACTACCAGGGTATCTAATCACTAGAGCTTGCTTTCCTGTAAAGATTGTC420.14508273170057687No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG420.14508273170057687No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG410.14162838094580124No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG410.14162838094580124No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT410.14162838094580124No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG400.1381740301910256No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA400.1381740301910256No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG390.13471967943624996No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCATGCCTCAG390.13471967943624996No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG380.1312653286814743No Hit
GACTACAGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT370.12781097792669868No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG360.12435662717192304No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG360.12435662717192304No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG360.12435662717192304No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA350.1209022764171474No Hit
GACTACCGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG340.11744792566237175No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTCTTCA330.11399357490759611No Hit
GACTACAAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT330.11399357490759611No Hit
GACTACCCGGGTATCTGCTTCTAAGCTCTCTCAGCTGATAGAACAAGGAA330.11399357490759611No Hit
GACTACCAGGGTATCTAATCCACACAGCAGAAATCAGAAACATCTGGTAT320.11053922415282047No Hit
GACTACCCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT320.11053922415282047No Hit
GACTACAGGGGTATCTAATCCCAAACGCTCTCCTCCATCGTCCCAGATGG310.10708487339804483No Hit
GACTACTGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT300.1036305226432692No Hit
GACTACCAGGGTATCTAATCAAACACCTACCATTGGTCTGGCACTCTCCT290.10017617188849356No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG290.10017617188849356No Hit
GACTACTCGGGTATCTAATCACTAGAGCTTGCTTTCCTGTAAAGATTGTC290.10017617188849356No Hit
GACTACACGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT290.10017617188849356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)