FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004835801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004835801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340
Sequences flagged as poor quality0
Sequence length39-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG205.88235294117647No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG185.294117647058823No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG154.411764705882353No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG144.117647058823529No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG144.117647058823529No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG123.5294117647058822No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG113.2352941176470593No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG113.2352941176470593No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG102.941176470588235No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG82.3529411764705883No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG82.3529411764705883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG72.0588235294117645No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG72.0588235294117645No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG72.0588235294117645No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG61.7647058823529411No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG61.7647058823529411No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA51.4705882352941175No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG51.4705882352941175No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG41.1764705882352942No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41.1764705882352942No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA41.1764705882352942No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG41.1764705882352942No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30.8823529411764706No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG30.8823529411764706No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG30.8823529411764706No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG30.8823529411764706No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30.8823529411764706No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG30.8823529411764706No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30.8823529411764706No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30.8823529411764706No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG30.8823529411764706No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA30.8823529411764706No Hit
NACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG30.8823529411764706No Hit
GACTACCAGGGTAACTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG30.8823529411764706No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20.5882352941176471No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG20.5882352941176471No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.5882352941176471No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20.5882352941176471No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG20.5882352941176471No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20.5882352941176471No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTACGCGCCTCAG20.5882352941176471No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20.5882352941176471No Hit
GATACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC20.5882352941176471No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20.5882352941176471No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.5882352941176471No Hit
GACTACACGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20.5882352941176471No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20.5882352941176471No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20.5882352941176471No Hit
GACACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGC20.5882352941176471No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTCGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACCGGGGTATCTAATCCTGTGTGCTCCCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10.29411764705882354No Hit
GGCTGCGGGGGTATGTAGTCCTGTTTGCTTCCGACGGTTTCGCGCCTTAG10.29411764705882354No Hit
GGCTACCAGGGGATCTGATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10.29411764705882354No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGCGCATCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTTCCCACGCTTTCGTGCATGAG10.29411764705882354No Hit
GACTACCAGAGTATCTAATCTGACGCAGCCTCCCGTAGG10.29411764705882354No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG10.29411764705882354No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.29411764705882354No Hit
GACTACCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAGC10.29411764705882354No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCACGAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAA10.29411764705882354No Hit
GACCTCCCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA10.29411764705882354No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10.29411764705882354No Hit
TACTACAACTTTCTCTCCTTCCAGTTTGTATCTTCGCTATACTGTGTTCA10.29411764705882354No Hit
GACTACTCGGGTTGCTAATCCTGTTTGTTCCCCCCACTTTCGATCCTCAG10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCCCCCCACGCTTTCGTGCCTCAG10.29411764705882354No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCTCGCTTTTGAGCTTTAG10.29411764705882354No Hit
CCCTAGCCGGGTAGCTGGTCCCGTCTGTTGTCCATGCGAACCAGCACGAA10.29411764705882354No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCTCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTCCTCGTGTCTCTAGTCCGAGAATGGTTTTTAAACTCAGAGCGTTTA10.29411764705882354No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.29411764705882354No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAA10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAGC10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCATCAG10.29411764705882354No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCACATCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGTTACCAACACATTCGACCATAAC10.29411764705882354No Hit
GAGTGCTAGGGTTTCTAATCCTGTGTGCTCCCCACGCTTTCGAGCTTCTA10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG10.29411764705882354No Hit
GACTACATGGCTGGCGCTTCGGCTCTGTTCTCGAGGATATCGTATGTCAA10.29411764705882354No Hit
GAACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATCAG10.29411764705882354No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAA10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10.29411764705882354No Hit
GACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCACGCCTTCGAGCCTCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG10.29411764705882354No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG10.29411764705882354No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCGCCTCAG10.29411764705882354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG10.29411764705882354No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA10.29411764705882354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)